摘要
目的:证实便塞通合剂对便秘模型大鼠的通便作用是通过调节血中GAS、MTL和SP等胃肠激素的分泌来实现的这一假说。方法:84只SD大鼠随机分为7组:空白组、模型组、阳性对照1组(麻仁丸组)、阳性对照两组(莫沙比利组)、便塞通合剂治疗高、中、低剂量组。用复方地芬诺酯复制便秘大鼠模型,确定模型建立后,各组分别给药,每天灌胃1次,7天为1疗程,疗程间停药1天,2个疗程后,收股动脉血采用ELISA法检测。结果:便塞通高、中剂量组和莫沙比利组大鼠的GAS、MTL和SP的含量显著增高,与模型组和空白组大鼠比较差异有极显著性(P<0.01);便塞通低剂量组和麻仁丸组大鼠的GAS和MTL含量与模型组大鼠比较有统计学差异(P<0.05);各给药组组间比较可见:便塞通高、中剂量组和莫沙比利组大鼠的GAS和MTL的含量高于便塞通低剂量组和麻仁丸组大鼠的含量,有统计学意义(P<0.05)。结论:便塞通合剂能提高大鼠血浆中GAS、MTL和SP等胃肠激素的含量,而具有通便作用,并且便塞通的通便作用皆呈一定的量效依赖关系,高、中剂量组作用优于低剂量组。
Objective：To confirm this hypothesis that the function of Biansetong mixture on constipation rats was regulated by gastrin（GAS）,motilin（MTL）,substance P（SP）.Methods：84 SD rats were randomly divided into seven groups：normal group,model group,Positive control group 1（MaRen Pills group）,positive control group 2（mosapride group）,high-dose BianSeTong mixture,middle-dose BianSeTong mixture and slight-dose BianSeTong mixture.Constipation rat model was established by Compound Diphenoxylate.After the success of modeling,every groups were given relevant theraperutic drugs respectively.Drugs were applied one time per day for 2 weeks.After a course of treatment（7 days）,the drugs were discontinuated 1 day.All animals were killed after 2 weeks.Then femoral artery blood were detected by enzyme linked immunosorbent assay（ELISA）.Results：The amount of GAS,MTL,SP in BianSeTong mixture groups it increased especially in middle-dose,high-dose group and mosapride group.They were significantly higher than model group and normal group（P0.01）;The amount of GAS,MTL In Maren Pills group and the low-dose BianSeTong mixture group was higher than model group（P0.05）.The amount of GAS,MTL in BianSeTong mixture middle-dose groups,high-dose group and mosapride group were significantly higher than that of Maren Pills group and the low-dose BianSeTong mixture group（P0.05）.Conclusion：Biansetong mixture can increase the amount of GAS,MTL and SP in the blood of constipation rat model.It＇s function can depend on dose,and high-dose BianSeTong mixture,middle-dose BianSeTong mixture were super to low-dose BianSeTong mixture group.
出处
《中华中医药学刊》
CAS
2011年第7期1549-1551,共3页
Chinese Archives of Traditional Chinese Medicine
基金
四川省杰出青年基金项目(06ZQ026-31)
