摘要
目的建立一种新生大鼠大脑皮层神经元原代培养方法。方法体外原代培养新生大鼠大脑皮质神经元,应用免疫细胞化学法鉴定神经元。结果用神经基础培养基（Neurobasal-A）＋B27培养的神经元纯度可达70%,生长状态较佳。结论该培养技术是大鼠皮层神经元体外培养的一种较理想的方法。
Objective To set up a method for primary culture of cerebral cortical neurons of newborn rats.Methods Primary culture of cerebral cortical neurons of newborn rats was made in vitro;immunocytochemical method was applied in identifying the cultured neurons.Result The neurons cultured with Neurobasal-A and B27 grew well and the purity reached 70%.Conclusions This method is an ideal technique for the culture in vitro cerebral cortical neurons of rats.
出处
《西南军医》
2011年第4期617-619,共3页
Journal of Military Surgeon in Southwest China
基金
四川教育厅项目：2006A155
关键词
大脑皮质
神经元
培养
cerebral cortex neurons culture
