摘要
目的：检测ｎｍ ２３ Ｈ１ 与ｍ ｕｃ１ 基因在乳腺癌中的表达水平，探讨ｎｍ ２３ Ｈ１ 与ｍ ｕｃ１ 在乳腺癌原发灶与转移灶中表达水平改变的临床意义． 方法：采用定量 Ｒ Ｔ Ｐ Ｃ Ｒ 与图像扫描定量分析技术检测２２ 例家族性乳腺癌及６０ 例散发性乳腺癌原发灶与转移灶中ｎｍ ２３ Ｈ１ 与 ｍ ｕｃ１ 基因的表达水平．结果：①在２２ 例有家族史的原发灶中， ｎｍ ２３ Ｈ１ 基因在１０ 例无转移的标本中检出率为８／１０，１２ 例转移淋巴结中检出率４／１２，癌旁组织中检出率为１４／２２；６０ 例散发性乳腺癌中，ｎｍ ２３ Ｈ１ 基因在４０ 例无转移的原发灶中检出率为 ３４／４０，２０ 例转移淋巴结中检出率为７／２０，癌旁组织中检出率为３９／６０；无转移的原发灶与转移灶中的检出率存在显著性差异（ Ｐ＜ ００１）；②ｎｍ ２３ Ｈ１ 基因在乳腺癌原发灶呈高表达，在转移灶中呈低表达，表达量在两者之间存在显著性差别（ Ｐ＜ ０．００１）；ｎｍ ２３ Ｈ１ 基因表达水平的高低与乳癌的分级、病理类型、家族史无关；③ ｍ ｕｃ１ 在２２ 例有家族史的无转移原发灶中检出率为２／１０，转移淋巴结中检出率为１０／１２，癌旁组织中检出率为 １５／２２；６０
AIM: To investigate the alteration of expression levels of nm23 H1 and muc1 in primary breast cancer and metastatic cancer, and to explore further the clinical significance of alteration of expression levels of nm23 H1 and muc1 . METHODS: By using quantitative RT PCR and Imagine Scanning Quantification Analysis System to examine the expression levels of nm23 H1 and muc1 in 22 samples of breast carcinoma with family history and 60 samples of dispersed breast carcinomas. RESULTS:①In 22 specimens of primary breast cancers with heredity family history, positive rates of nm23 H1 were respectively 8 /10 in primary breast cancers without metastasis, 4/12 in metastasis nodes, 14/22 in paracancer tissues ; in 60 specimens of dispersed breast cancers, positive rates of nm23 H1 were respectively 34/40 in primary breast cancers without metastasis,7/20 in metastasis nodes, 39/60 in paracancer tissues;there were significantly differences between positive rates of nm23 H1 in primary tumors and ones in metastasis tumors ( P< 0.01); ②Expressional level of nm23 H1 was higher in primary breast cancers than in metastatic tissues, there was a significant difference between expressional levels in primary breast cancer and ones in metastatic tumors ( P< 0.01); expressional levels of nm23 H1 had no correlated with degrees of cancers and pathological types and heredity family history; ③In 22 specimens of primary breast cancers with heredity family history, rates of muc1 were respectively 2 /10 in primary breast cancers without metastasis, 10/12 in metastatic nodes, 15/22 in paracancer tissues ; in 60 specimens of dispersed breast cancers, rates of muc1 were respectively 10/40 in primary breast cancers without metastasis, 16/20 in metastatic nodes, 45/60 in paracancer tissues;there were significant differences between rates of muc1 in primary tumors and ones in metastatic tumor
出处
《第四军医大学学报》
1999年第9期823-826,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金
