摘要
目的探讨肝大部分切除(PH)诱导的肠源性内毒素血症(IETM)对血浆及残余肝组织IL-1β的影响或可能存在的关系。方法 102只健康雄性成年wistar大鼠随机分为正常对照(NC)组、假手术(SO)组和肝大部切除(PH)组,在不同时间点检测血浆内毒素(ET)、IL-1β、ALT的水平以及残余肝组织IL-1β的蛋白表达。结果血浆ET在PH术后6～72h期间升高,在12h、48h呈现峰值,分别与NC组比较(P<0.05,P<0.01);血浆ET在SO组各时间点与NC组比较差异无显著性。血浆IL-1β在PH后6～72h期间升高,与血浆ET动态变化趋势呈显著正相关(r=0.729,P<0.05)。SO组在术后各个时间点与NC组比较差异无显著性。血浆ALT在PH组术后6～72h期间升高,与血浆ALT动态变化趋势呈显著正相关(r=0.767,P<0.05)。PH组残余肝组织IL-1β术后6h于肝细胞质表达,24h IL-1β于肝细胞膜表达。48h～72h IL-1β于肝细胞膜的肝窦面表达且强度明显增加。72h后IL-1β的表达明显减少,至168h IL-1β表达完全消失。NC组肝组织内IL-1β阴性,SO组术后各时间点未见IL-1β特异性染色。结论 PH后诱导的肠源性内毒素直接影响IL-1β的生成变化,两者变化趋势呈显著正相关;IETM可能通过IL-1β的生物学作用影响肝再生。
Objective To compare and evaluate the dynamic changes between endotoxemia and IL -1β after partial hepatectomy （PH） in rats. Methods One- hundred and two adult male wistar rats were randomized into three groups： normal control （NC） group, PH group and sham-operated （SO） group. To study the dynamic changes of plasma endotoxemia, IL-1β, ALT levels and residual liver protein expression of IL-1β. Results Plasma ET levels significantly increased during 6-72 h after PH, there were two peak values at 12,48 h. And compared respectively with the ET levels of NC group （P〈0.05, P〈 0.01 ）. Plasma IL-1β levels were significantly increased during 6-72 h after PH. There was positive relationship between the dynamic changes of IL-1β and ET in plasma （r=0. 729, P〈0.05）. Plasma ALT levels significantly increased during 6-72 h period dynamic time points after PH, and there was positive relationship between the dynamic changes of IL-1β and ALT （r = 0. 767, P〈0.05）. P H group residual liver tissue after 6hIL-1β expression in the liver cytoplasm, 24 h IL-1β expression mainly in the liver cell membrane. 48 h- 72 h IL-1β expression in liver sinusoidal membrane surface and the intensity increased significantly. After 72 h the expression of IL-1β significantly reduced to 168 h IL-1β expression disappeared completely. NC group IL-1β in liver tissue negative, SO group was no specific staining at all time points. Conclusion Endotoxemia induced by PH can directly affect the generation of IL-1β, and there was positive relationship between the dynamic changes of IL-1β arid ET. IETM may affect liver regeneration through the biological role of IL-1β.
出处
《国际消化病杂志》
CAS
2011年第3期172-176,共5页
International Journal of Digestive Diseases
