摘要
目的观察黄芪苷IV对异丙肾上腺素(isoproteronol,ISO)诱发的小鼠心肌缺血损伤的影响.方法采用皮下注射ISO制造心肌缺血模型,记录小鼠标Ⅱ导联心电图,用J点变化评价心肌损伤,并记录注射ISO后5 min内的心律失常发生情况;取血,以羟胺法测定SOD活性;快速断头,观察小鼠耐急性缺氧的能力;取心肌观察其超微结构的变化.结果与模型组比较,黄芪苷IV能明显降低J点的抬高程度(1.83±1.1)mV vs(13.15±1.24)mV,P<0.01;减少缺血后室性心率失常的发生(2.1±1.3)vs(12.4±3.8),P<0.01;升高SOD活性(378±66)NU.mL-1vs(311±46)NU.mL-1,P<0.05;提高小鼠耐急性缺氧的能力(28.6±2.5)s vs(16.4±2.4)s,P<0.05;并能显著缓解ISO所导致的心肌超微结构的损伤.结论黄芪苷IV对ISO诱发的小鼠心肌缺血损伤具有保护作用.
Objective To observe the effects of Astragaloside IV on myocardial ischemia injury induced by isoproterenol（isoproteronol,ISO）in mice.Methods Myocardial ischemia model was made by subcutaneous injection of ISO.The extent of myocardial damage was evaluated with J-point of Ⅱ ECG and ventricular arrhythmias were recorded within 5min after isoprenaline was injected.SOD activity was detected with hydroxylamine method;the ability of resistance to acute hypoxia was observed through fast decapitation;while heart was taken to observe the ultra structure changes.Results Astragaloside IV could significantly reduce increases of J-point（1.83±1.1 vs 13.15±1.24,P0.01）（mV）and the ventricular arrhythmia（2.1±1.3 vs 12.4±3.8,P0.01）;elevate SOD activity（378±66 vs 311±46,P0.05）（NU.ml-1）;raise resistance to the ability of acute hypoxia（28.6±2.5 vs 16.4±2.4,P0.05）（s）;and could significantly improve the ISO myocardial ultra structure change.Conclusion Astragaloside IV has protective effect on ISO-induced myocardial ischemic injury in mice.
出处
《河北北方学院学报（自然科学版）》
2011年第3期59-62,65,共5页
Journal of Hebei North University:Natural Science Edition
