摘要
目的研究N-乙酰半胱氨酸(NAC)与哌拉西林、环丙沙星、阿米卡星对铜绿假单胞菌的协同抑菌作用。方法应用微量肉汤稀释法测定最低抑菌浓度(MIC);采用棋盘法检测NAC与哌拉西林、环丙沙星、阿米卡星对铜绿假单胞菌的联合抑菌作用,计算最小部分抑菌浓度指数。结果 NAC对大多数铜绿假单胞菌MIC在10-40mg/ml。对33.3%的铜绿假单胞菌,NAC与哌拉西林联合应用存在协同抑菌作用;对50%的铜绿假单胞菌,NAC与环丙沙星联合应用存在协同抑菌作用;NAC与阿米卡星联合应用对铜绿假单胞菌存在无关(86.7%)或拮抗作用(13.3%)。结论 NAC对铜绿假单胞菌有抑菌作用,并与哌拉西林、环丙沙星存在一定协同抑菌作用。
Objective To study the synergistic inhibitory effect of N-acetylcysteine （NAC） in combination with piperacillin, ciprofloxacin and amikacin on Pseudomonas aeruginosa strains. Methods Minimum inhibitory concentrations（MICs） of NAC and three antibiotics were measured by broth microdilution test. Synergistic inhibitory effect of NAC in combination with piperacillin, ciprofloxacin and amikacin was determined by broth checkerboard assay. Fractional inhibitory concentration index was calculated. Results The MIC of NAC against most Pseudomonas aeruginosa strains was 10-40mg/ml. The NAC in combination with piperacillin, ciprofloxacin showed a synergistic effect against 33.3% and 50% Pseudomonas aeruginosa strains, respectively. The NAC in combination with amikacin demonstrated no synergistic effect or antagonism against Pseudomonas aeruginosa strains. Conclusion NAC in combination with piperacillin and ciprofloxacin has an synergistic inhibitory effect against Pseudomonas aeruginosa strains.
出处
《军医进修学院学报》
CAS
2011年第7期684-686,共3页
Academic Journal of Pla Postgraduate Medical School
