摘要
目的:探讨外源性5'-核苷酸对大鼠急性乙醇中毒的影响。方法:将24只雄性SD大鼠随机分成4组:生理盐水组、低剂量核苷酸组、中剂量核苷酸组和高剂量核苷酸组。乙醇染毒前30 min,各组分别用生理盐水、低剂量(0.2 g/kg体重)、中剂量(0.8 g/kg体重)、高剂量(3.2 g/kg体重)核苷酸灌胃,乙醇染毒剂量为3 g/kg体重,染毒后进行旷场实验、转棒实验,检测血清乙醇浓度、血清谷草转氨酶、谷丙转氨酶、总甘油三酯、总胆固醇、高密度脂蛋白胆固醇、白蛋白、总蛋白水平,超氧化物歧化酶活性和丙二醛含量,检测肝乙醇脱氢酶活性,评价核苷酸对急性乙醇中毒的影响。结果:外源性5'-核苷酸干预能够使大鼠血液乙醇浓度降低,高剂量核苷酸组与生理盐水组相比[(0.56±0.18 g/L)vs.(1.11±0.44 g/L),P<0.05],差异具有统计学意义,低剂量核苷酸组(1.04±0.35 g/L)和中剂量核苷酸组(0.93±0.14 g/L)与生理盐水组相比也较低,但差异无统计学意义;各组肝乙醇脱氢酶活性差异无统计学意义,各组大鼠神经行为学表现、血清生化水平和抗氧化水平差异也无统计学意义。结论:外源性5'-核苷酸干预对大鼠急性乙醇中毒无明显影响。
Objective：To investigate effects of exogenous 5′-nucleotides on acute alcohol intoxication in SD rats.Methods：In our study,24 male SD rats were randomly divided into 4 groups which included a control group treated with normal saline and three experimental groups treated with low,medium and high doses of exogenous 5′-nucleotides（0.2,0.8,3.2 g/kg body weight）.All the rats were gavaged with 50% ethanol 30 minutes after treatment.Then rotarod test and open field test were taken to assess rats＇ neurobehavior changes;Tail blood samples were collected to test blood ethanol concentration;Then all the rats were anesthetized and killed to collect blood and liver samples.Contents of serum alanine amino transferase,aspartate amino transferase,triglyceride,total cholesterol,high density lipoprotein cholesterol,total protein and albumin were tested;Their serum superoxide dismutase activity,malondialdehyde content and liver alcohol dehydrogenase activity were measured.Results：Compared with the controls,high dose nucleotides treated rats had lower serum ethanol concentration [（0.56±0.18 g/L）vs.（1.11±0.44 g/L）,P〈0.05].However,exogenous 5′-nucleotides had no impact on neurobehavior and serum biochemical indicators;No difference was found in liver alcohol dehydrogenase activity,serum superoxide dismutase activity and malondialdehyde content were also found no different between the groups.Conclusion：Exogenous 5′-nucleotides have no protective properties for acute alcohol intoxication in rats.
出处
《北京大学学报（医学版）》
CAS
CSCD
北大核心
2011年第3期338-341,共4页
Journal of Peking University:Health Sciences
关键词
核苷酸
酒精中毒
急性
神经行为学表现
Nucleotides； Alcoholic intoxication； Neurobehavioral manifestations
