摘要
目的 探讨卡维地洛和黄芪对心力衰竭（简称心衰）时兰尼碱受体/钙释放通道（ryanodine receptor,RyR）的作用.方法 采用腹主动脉缩窄术建立幼鼠心衰模型,术后6周随机分为3组：心衰组、卡维地洛治疗组和卡维地洛＋黄芪治疗组,另设假手术对照组,每组30只.卡维地洛、黄芪灌胃给药,术后每日观察幼鼠的呼吸、皮毛颜色、活动量、体重等发育状况.4周后行高频超声检测,超速离心分离肌质网（SR）,荧光分光光度仪检测钙离子（Ca2＋）的重吸收和渗漏.逆转录-聚合酶链反应（RT-PCR）测定RyR2 mRNA的表达.结果 与假手术组比较,心衰组幼鼠左室舒张末期内径（LVEDD）（P＜0.05）、左室收缩末期内径（LVESD）、室间隔舒张末期厚度（IVSTd）、室间隔收缩末期厚度（IVSTs）、左室后壁舒张末期厚度（LVPWTd）、左室后壁收缩末期厚度（LVPWTs）均高（P＜0.01）,短轴缩短率（FS）、射血分数（EF）均低（P＜0.01）;与心衰组比较,卡维地洛治疗组、卡维地洛＋黄芪治疗组左室、右室相对质量低,LVEDD（P＜0.05）、LVESD、IVSTd、IVSTs、LVPWTd、LVPWTs均低（P＜0.01）,FS、EF均高（P＜0.01）.卡维地洛＋黄芪治疗组较卡维地洛治疗组体重高（P＜0.05）,FS（%）（心衰组44.55±1.20,卡维地洛治疗组44.55±1.20,卡维地洛＋黄芪治疗组53.58±1.30,假手术组59.03±1.67）、EF（%）（心衰组51.60 ±1.15,卡维地洛治疗组72.06 ±1.39,卡维地洛十黄芪治疗组79.06 ±1.09,假手术组85.86 ±1.45）（P＜0.05）均高.分别向四组SR的缓冲液中仅加入thapsigargin,心衰组（65.6±6.2）出现较假手术组（11.5 ±4.3）、卡维地洛治疗组（15.6±5.8）及卡维地洛＋黄芪治疗组（13.6 ±4.8）明显的Ca2＋渗漏（%）（P＜0.01）;而当thapsigargin和FK506-起加入四组sR的缓冲液中,心衰组（67.3±7.5）、假手术组（60.6 ±7.8）、卡维地洛治疗组（66.2±4.5）、卡维地洛＋�
Objective To explore change of ryanodine receptor（RyR）in junior mouse with heart failure（HF） and the effect of β-adrenoreceptor blocker and Radix astragali on RyR in HF in this experiment.Method The animal model of congestive heart failure was established by coarctation of abdominal aorta. Five weeks old mice were randomly divided into 4 groups：（1）HF group without treatment （n=30）;（2）HF group treated with earvedilol（n=30）;（3）HF group treated with carvedilol and Radix astrali（n=30）;（4）Sham-operated group（n=30）.Carvedilol and Radix astragali were administered through direct gastric garage.After 4 weeks of treatment the high frequency ultrasound was performed.Myocardial sarcoplasmic reticulum（SR）was fractionated with ultra centrifugation.The time courses of Ca2＋uptake and leak were determined by fluorescent spectrophotometry.The levels of expression of RyR2 in the 4groups were detected by semi-quantitative reverse transcription-polymerase chain reaction. Resuit Compared with the sham-operated group,left ventricular diastolic dimension（LVEDD）（P＜0.05）,left ventricular svstolic dimension （LVESD）, interventricular septal thickness at end-diastole（IVSTd）,interventricular septal thickness at end-systole（IVSs）,left ventrieular posterior wall thickness at enddiastole（LVPWTd）,and left ventricular posterior wall thickness at endsystole（LVPWTs）were all significantly increased（P＜0.01）,ejection fraction（EF）（%）（HF group without treatment 51.60±1.15,HF treated with carvedilol 72.06±1.39.HF treated with earvedilol and Radix astragali 79.06±1.09,sham-operated group 85.86±1.45）and fractional shortening（FS）（HF group without treatment 44.55±1.20.HF treated with carvedilol 44.55±1.20,HF treated with carvedilol and Radix astragali 53.58 ±1.30.sham-operated group 59.03±1.67）were decreased（P＜0.01）in HF group without treatment.LVEDD（P＜0.05）,LVESD,IVSTd,IVSTs,LVPWTd and LVPWTs were all significantly decreased（P＜0.0
出处
《中华儿科杂志》
CAS
CSCD
北大核心
2011年第6期433-438,共6页
Chinese Journal of Pediatrics
关键词
卡唑类
丙醇胺类
黄芪
兰尼碱受体钙释放通道
心力衰竭
充血性
Carbazoles
Propanolamines
Astragalus membranaceus
Ryanodine receptor caleium release channel
Hcart failure,congestive
