摘要
为探讨水牛易感大片吸虫的免疫机理,采用Ficoll-Hypaque密度梯度离心法分离水牛外周血单个核细胞,再经贴壁法纯化单核/巨噬细胞,用Geriss法检测在大片吸虫分泌排泄产物(ESP)或脂多糖(LPS)作用下,水牛外周血单核/巨噬细胞一氧化氮(NO)的表达量。结果显示,大片吸虫ESP或LPS都可单独作用刺激水牛外周血单核/巨噬细胞产生NO。大片吸虫ESP(0.1、1μg/mL)和LPS(0.1μg/mL)联合作用时水牛外周血单核/巨噬细胞产生NO的量低于LPS(0.1μg/mL)单独作用时的量,差异显著(P<0.05)。大片吸虫ESP(10μg/mL)和LPS(0.1μg/mL)联合作用时水牛外周血单核/巨噬细胞产生NO的量与LPS(0.1μg/mL)单独作用时差异不显著(P>0.05)。结果表明,大片吸虫ESP或其中组分能抑制水牛外周血单核/巨噬细胞产生NO的作用,这可能是水牛易感大片吸虫的一个原因。
In order to study the immune mechanism of the susceptibility to Fasciola gigantica in buffalo,mononuclear cells were obtained from buffalo peripheral blood by the method of Ficoll-Hypaque density gradient centrifugation.Macrophages were enriched by adherence.The NO production by excretory and secretory products（ESP） and F.gigantica or lipopolysaccharide（LPS）-stimulated peripheral blood mononuclear cell（PBMC） / macrophage cell（MC）was evaluated by Geriss method.NO was produced by ESP or LPS-stimulated respectively.The NO production stimulated by ESP（0.1 and 1 μg/mL） combined with LPS（0.1 μg/mL） in PBMC/MC was significantly lower than that stimulated by LPS（0.1 μg/mL）（P〈0.05）.The NO production by ESP（10 μg /mL）-stimulated PBMC/MC was not significantly different to that LPS（0.1 μg/mL）-stimulated（P〉0.05）.These results showed that ESP or other component may suppress the NO production by PBMC/MC,and might be partly responsible for buffaloes being highly susceptible to F.gigantica.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2011年第6期620-624,共5页
Chinese Veterinary Science
基金
广西自然科学基金项目(0832036)
国家自然科学基金项目(30860204)
