摘要
目的:了解深圳市龙岗区海产品、淡水产品及外环境水体中致病性弧菌分布状况。方法:采集深圳市龙岗区各酒店、超市、海鲜集贸市场等常见的海产品和淡水产品以及外环境水体共506份,通过实时荧光PCR法初筛后采用常规的增菌、细菌分离培养和鉴定方法进行。副溶血性弧菌产毒基因用PCR方法检测。结果:506份样品中共分离到副溶血性弧菌188株(37.2%),其中1株带tdh基因,非致病的霍乱弧菌74株(14.6%),其它弧菌42株(8.3%)。结论:要加强对水产品及外环境水体卫生监督管理。
Objective：To know the distribution of pathogenic Vibrio spp.in marine products,freshwater products and external environmental water in Longgang district,Shenzhen.Methods： Five hundred and six samples were collected from common marine products,freshwater products and external environmental water at hotels,supermarkets and seafood markets in Longgang district,Shenzhen.Samples were preliminary screened by real-time PCR.Then they were enriched,isolated and identified by routine methods.The virulence factor of Vibrio parahaemolyticus was detected by PCR methods.Results： One hundred and eight（37.2%） V.parahaemolyticus,74（14.6%） non-pathogenic V.cholerae and 42（8.3%） other pathogenic Vibrio spp.were isolated in 506 samples.One V.parahaemolyticus with tdh virulence gene was detected.Conclusion： The surveillance and management of aquatic products and ex-enviromental water should be strengthened.
出处
《中国卫生检验杂志》
CAS
2011年第6期1512-1514,共3页
Chinese Journal of Health Laboratory Technology
基金
2009年度深圳市龙岗区科技局立项课题(YL-2009053)
关键词
致病性弧菌
水产品
外环境水体
Pathogenic Vibrio spp.
Aquatic products
Ex-environmental water
