摘要
目的:了解宁波市手足口病的病原学特征,为手足口病的综合防治提供科学依据。方法:收集2009年-2010年宁波市手足口病临床诊断病例的相关标本,采用荧光定量RT-PCR或RT-PCR进行病原学检测。结果:在446份临床标本中,共检测到阳性标本346份,总检出率为77.58%,男女比例为2.035∶1,手足口病发病高峰在春末和夏初,其中EV71阳性标本191份;CoxA16阳性标本121份;CoxA6阳性标本13份;CoxA10阳性标本7份;CoxA12阳性标本4份;ECHO9阳性标本4份;CoxA2阳性标本2份;CoxA4、CoxB1、CoxB4和其它肠道病毒各1份,EV71是引起宁波市手足口病的优势毒株(P<0.05)。结论:宁波市手足口病的病原体主要是EV71(55.20%)和CoxA16(34.98%),同时呈现多样化和复杂化的特点。EV71是引起手足口病重症病例和死亡病例的主要毒株类型。
Objective：To investigate epidemiological features of the pathogen of HFMD in Ningbo,which may provide reference for preventing and controlling the HFMD.Methods： 446 samples were collected from clinically diagnosed cases with HFMD,real-time quantitative RT-PCR or RT-PCR were carried out for nucleinic acid identification and typing.Results： Among the 446 clinical samples,346（77.58%）cases were tested positive,the proportion of male and female in the cases of HFMD was 2.035∶ 1.The peak of HFMD was in late spring and early summer.There were 191 cases of EV71 positive;121 cases of CoxA16 positive;13 cases of CoxA6 positive;7 cases of CoxA10 positive;4 cases of CoxA12 and ECHO9 positive;2 cases of CoxA2 positive;1 cases of CoxA4、CoxB1、CoxB4 and other enterovirus positive.EV71 was the dominant pathogen of the HFMD in Ningbo in 2009-2010（P0.05）.Conclusion： The HFMD was mainly caused by EV71（55.20%）and CoxA16（34.98%）in Ningbo and showed characteristics of diversified and complicated.EV71 was the major enterovirus causing HFMD severe cases.
出处
《中国卫生检验杂志》
CAS
2011年第6期1484-1486,共3页
Chinese Journal of Health Laboratory Technology
基金
浙江省卫生厅医药卫生科学研究基金资助项目(2009A190)
宁波市科技局重大科技专项资助项目(2009C50008)
