摘要
目的:分析大连市2005年-2009年流感病毒H1N1亚型血凝素抗原性及其基因变异情况,为流感防治提供技术支持。方法:采集流感样病例的鼻咽拭子标本,用MDCK细胞进行流感病毒的病原分离,采用红细胞凝集实验测定病毒的滴度,用血凝抑制试验和RT-PCR进行流感病毒型别鉴定,通过RT-PCR扩增血凝素HA片段的基因,电泳、纯化后进行基因序列测定,利用生物信息学进行序列分析。结果:2005年-2009年共分离到季节性甲型H1N1流感病毒39株,其中28株进行了测序。与当年的疫苗株相比,2005年、2006年、2007年和2008年分离株的HA1区分别发生了11个、9个、15、15个突变。结论:在近几个年度的流行季节中,该地区有H1N1亚型流感的存在。该地H1N1流感病毒分离株有多处氨基酸发生替换,氨基酸的替换导致毒株的抗原性发生了漂移。
Objective：To analyze the data of influenza A H1N1 viruses surveillance and genetic characteristics from Dalian City during 2005 –2009,in order to provide a scientific basis for the prevention and treatment of influenza.Methods： The specimens from Influenza-Like Illness（ILI） were collected.The viruses were isolated with MDCK cell and identified with HA and RT-PCR.The products of PCR were sequenced.Then the sequences were analyzed by biometric software.Results： A total of 39 influenza strains were identified as A（H1N1）subtype,and 28 of them were sequenced.Compared with standard sequences,there were 11,9,15,15 mutations in the amino acid sequences of virus strains isolated in 2005,2006,2007 and 2008 respectively.Conclusion： Influenza type A H1N1 are detected in Dalian City.There are several mutations in the amino acid sequences of virus strains isolated in Dalian.The antigenic drift of virus strains is due to accumulation of amino acid substitutions.
出处
《中国卫生检验杂志》
CAS
2011年第6期1426-1428,共3页
Chinese Journal of Health Laboratory Technology
基金
大连市流感病毒血凝素(HA)基因遗传特性研究(2007E21SF202)
