摘要
目的:用碳纳米管-离子液体纳米流体修饰玻碳电极,构建测定尿酸的电化学传感器。方法:将构建的电化学传感器置于尿酸的磷酸盐缓冲溶液中,实验结果表明,尿酸在修饰电极上产生一氧化峰,峰电位为0.570 V(vs.SCE),较裸电极负移0.170 V,且峰电流大大增强。据此,建立了测定UA的电化学分析方法。结果:在选定的最佳实验条件下,用线性扫描循环伏安法(LSV)测定尿酸的线性范围为5×10-6mol/L^8×10-4mol/L,检出限为5.5×10-7mol/L。结论:该方法可直接用于人体尿液中UA的测定,结果满意。
Objective：To build a new chemical sensors for detecting uric acid（UA） by using a CNTs-ionic liquid nanofluid modified electrode.Methods： In a phosphate buffer solution containing uric acid,the oxidation peak potential of uric acid was about 0.570 V（vs.SCE） at the modified electrode,the peak potential of uric acid shifted negatively for 0.170 V and the peak current increased obviously.So an electrochemical method for the determination of citric acid was established.Results： Under optimal experimental conditions,The linear range for the determination of UA was 5×10-6-8×10-4 mol/L by linear sweep voltammetry（LSV） and the detection limit was 5.5×10-7 mol/L.Conclusion： The method can be used directly for the determination of UA in human urine with satisfactory results.
出处
《中国卫生检验杂志》
CAS
2011年第6期1344-1346,共3页
Chinese Journal of Health Laboratory Technology
基金
安徽省高等学校省级自然科学研究项目(KJ2010A305)
安徽省科技厅自然科学基金项目(11040606M42)
关键词
尿酸
纳米流体
化学修饰电极
伏安法
Uric acid
Nanofluid
Chemical modified electrode
Voltammetry
