摘要
目的应用吉非替尼作用于鼻咽癌细胞株CNE2,观察其放疗增敏作用。方法体外培养鼻咽癌细胞株CNE2,以MTT实验检测细胞增殖抑制情况及吉非替尼对细胞放射敏感性的影响;用克隆形成法绘制细胞存活曲线,获得不同处理条件下的放射增敏比。以流式细胞术(FCM)分析细胞周期变化和凋亡情况。结果 MTT显示与单纯照射组比较,联合吉非替尼照射组细胞生存率显著降低(0.582±0.012vs0.398±0.016,P=0.0020);FCM显示吉非替尼联合放射组S期细胞显著下降(P=0.000),而G2/M期细胞比值显著增加(P=0.000),吉非替尼和放射线可协同作用,使CNE2细胞的周期再分布显著变化。结论吉非替尼可增强鼻咽癌细胞株CNE2放射敏感性,其机制可能与改变细胞周期的分布相关。
Objective To study the radiosensitizing effect of gefitinib on nasopharyngeal carcinoma cell line CNE2 in vitro. Methods Nasopharyngeal carcinoma cell line CNE2 was cultured in RP2MI 1640. MTT assay was performed to evaluate the cell proliferation changes in response to gefitinib treatment and the radiosensitizing effect of gefitinib. The cell survival curves and sensitive enhancement ratio （SERs） were obtained with a clonogenic assay. Flow cytometry analysis was applied to detect the cell cycle changes and cell apoptosis. Results MTT assay showed that cells exposed to gefitinib and radiation had a significantly lower survival ratio compared to the cells with radiation exposure only （0.582±0.012 vs 0.398±0.016, P=0.002）, with a SER of 1.535±0.134. The S phase cell percentage was significantly decreased and G2-M phase cells increased in gefitinib plus radiation group （P=0.000）, suggesting a synergistic effect of gefitinib and radiation. Conclusion Gefitinib can enhance the radiosensitivity of nasopharyngeal carcinoma CNE2 cells in vitro possibly by inhibiting cell proliferation, inducing cell apoptosis, and causing changes in the cell cycle distribution
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2011年第6期991-994,共4页
Journal of Southern Medical University
基金
国家自然科学基金(30973846)
广东省自然科学基金博士启动项目(8451051501001344)~~
关键词
吉非替尼
放射增敏
细胞周期
细胞凋亡
鼻咽癌细胞
gefitinib
radiosensifivity
cell cycle
apoptosis
nasopharyngeal carcinoma cells
