摘要
目的:研究Ⅰ型糖尿病(DM)小鼠心肌缺血/再灌注(I/R)损伤的变化,以及胰岛素(ISL)治疗控制血糖对Ⅰ型DM小鼠心肌I/R损伤的影响。方法:72只健康雄性昆明小鼠以链脲佐菌素(STZ)腹腔注射制备Ⅰ型DM模型,建模后用ISL治疗控制血糖2周。实验分为假手术(sham)组、I/R组、DM+I/R组、DM+ISL+I/R组,每组18只。采用结扎冠状动脉左前降支30 min制备心肌梗死(MI)模型,分别于再灌注后3 h,用TUNEL法检测心肌细胞凋亡,用ELISA法检测caspase-3活性、硝基酪氨酸的含量。再灌注后24 h,用TTC染色法检测MI面积。结果:与I/R组相比,DM+I/R组MI面积增大、心肌细胞凋亡数量增加、caspase-3活性升高、硝基酪氨酸的含量增加(P<0.01)。与DM+I/R组相比,DM+ISL+I/R组心肌细胞凋亡数量减少(P<0.01),MI面积减少、caspase-3活性降低、硝基酪氨酸含量减少(P<0.05)。结论:Ⅰ型DM小鼠心肌I/R损伤增加,ISL治疗控制血糖可以减轻其心肌I/R损伤。
AIM ： To investigate the effect of glucose control with insulin on myocardial ischemia/reperfusion （MI/R） in streptozotocin （STZ）-induced diabetic mice. METHODS： Type 1 diabetes was induced by i.p. injection of STZ and was treated with insulin for 2 weeks. Mice were divided into four groups： sham group, I/R group, DM ＋ I/R group, and DM ＋ insulin ＋ I/R group. Myocardial ischemia was produced by temporarily exteriorizing the heart via a left thoracic incision and placing a 6-0 silk suture slipknot around the left anterior descending coronary artery. After 30 min of ischemia, the slipknot was released and the myocardium was reperfused for 3 h （for caspase-3 activity and nitrotyrosine contents by ELISA, apoptosis by TUNEL） or 24 h （for infarct size by TTC staining）. RESULTS： Compared with those in I/R group, infarct size, myocardial apoptosis, caspase-3 activity and nitrotyrosine contents increased in DM ＋ I/R group （P 〈 0. 01 ）. Compared with those in DM ＋ I/R group, myocardial apoptosis （ P 〈 0. 01 ）, infarct size, caspase-3 activity and nitrotyrosine contents （ P 〈 0.05 ） were reduced in DM ＋ insulin ＋ I/R group. CONCLUSION： Type 1 diabetes aggravates M1/R injury and glucose control with insulin attenuates MI/R injury in STZ-induced diabetic mice.
出处
《心脏杂志》
CAS
2011年第3期284-288,共5页
Chinese Heart Journal
基金
国家自然科学基金项目资助(30670879)
西京医院学科助推计划项目资助(Xjzt08z02)
关键词
胰岛素
糖尿病
缺血/再灌注损伤
小鼠
insulin
glucose control
diabetes
ischemia/reperfusion injury
mice
