摘要
目的制备壳聚糖/β-甘油磷酸钠(CS/β-GP)膜,通过体外细胞培养评价新型引导组织再生膜的细胞生物相容性。方法利用CS/β-GP体系的温敏相转变特性,通过分子自组装技术合成温敏凝胶膜,采用红外光谱分析、扫描电镜、拉伸强度实验进行结构和力学测试。通过MTT比色法对体外培养的小鼠成纤维细胞L929生长及增殖情况进行初步评价,共分3组进行对比研究:实验A组(2%CS+0.5 gβ-GP)、实验B组(2%CS+1.0 gβ-GP)及空白对照组。结果 红外光谱分析提示,壳聚糖与β-甘油磷酸钠分子间存在静电作用和化学键结合,形成新的化合物;扫描电镜观察,壳聚糖/β-甘油磷酸钠膜具有多孔的表面结构和内部结构;L929细胞体外培养第3、4、5天,实验组与空白对照组吸光度(A)值组间差异无统计学意义(P>0.05)。结论 壳聚糖/β-甘油磷酸钠温敏凝胶具有良好的成膜性,能促进成纤维细胞的体外增殖,是一种有应用前景的新型引导组织再生膜材料。
Objective To observe the film-forming property and evaluate the cellular biocompability of chitosan/β-Glycerophosphate salt （CS/β-GP） thermo-sensitive hydrogel membrane for guided tissue regeneration（GTR）. Methods A new composite membrane was synthesized by molecular self-assembly technique using the thermal phase inver- sion property of the CS/β-GP system. The chemical structure of the membrane was analyzed by infrared spectrometer and the tensile strength was measured by a universal testing machine. The proliferation of mouse fibroblasts （ L929 cells） in vitro was preliminarily evaluated by MTT assay, which was categorized into 3 groups： experimental group A （2% CS ＋0.5gβ-GP）, experimental group B（2% CS ＋ 1.0gβ-GP） and the control group. Results Infrared spectrum analysis suggested that a new composite was formed through the chemical bonds between CS and β-GP molecules. The tensile strength of the membrane slightly decreased with an increase of β-GP. MTT assay showed that the difference between the A value of the experimental group and that of the control group on day 3,4 and 5 when cultured with L929 cells was not significant（P 〉 0.05）. Conclusion The CS/β-GP composite demonstrates a film-forming property and good cellular biocompability which should be a promising biomaterial for GTR membrane fabrication.
出处
《山东大学学报（医学版）》
CAS
北大核心
2011年第6期80-83,88,共5页
Journal of Shandong University:Health Sciences
关键词
壳聚糖
β-甘油磷酸钠
引导组织再生膜
温敏凝胶
细胞生物相容性
Chitosan
β－Glycemphosphate salt
Guided tissue regeneration membrane
Thermosensitive hydrogel
Cellular biocompatibility
