摘要
【目的】研究L-肉碱强化卤虫对鲤鱼开口苗脂肪酸组成和C/N比率的影响,为鲤鱼开口苗的饲养提供参考。【方法】分别用质量浓度为0(对照),1,100,1 000 mg/L的L-肉碱强化卤虫无节幼体(Artemiasp.)12和24h,然后投喂给鲤鱼(Cyprinus carpio)开口苗,21 d后测定鱼苗的脂肪酸组成和C/N比率。【结果】投喂L-肉碱强化12 h的卤虫时,1 mg/L L-肉碱处理组鲤鱼开口苗的∑C14-24脂肪酸含量、饱和脂肪酸含量和单不饱和脂肪酸含量较对照组显著降低(P<0.05);1 000 mg/L L-肉碱处理组鲤鱼开口苗的单不饱和脂肪酸含量较对照组显著升高(P<0.05);L-肉碱对鲤鱼开口苗的多不饱和脂肪酸含量、∑n-3+∑n-6和∑DHA+EPA含量均无显著影响(P>0.05);1 000mg/L L-肉碱处理组鲤鱼开口苗的C/N比率显著低于对照组和1 mg/L L-肉碱处理组(P<0.05)。投喂L-肉碱强化24 h的卤虫时,1和100 mg/L L-肉碱处理组鲤鱼开口苗的∑C14-24脂肪酸和饱和脂肪酸含量较对照组显著降低(P<0.05),但2组之间差异不显著(P>0.05);1 000 mg/L L-肉碱处理组鲤鱼开口苗的∑C14-24脂肪酸和饱和脂肪酸含量较对照组显著升高(P<0.05);1 mg/L L-肉碱处理组鲤鱼开口苗的单不饱和脂肪酸含量较其他3组显著降低(P<0.05),多不饱和脂肪酸、∑n-3+∑n-6和∑DHA+EPA含量与对照组差异不显著(P>0.05);100 mg/L和1 000 mg/L L-肉碱处理组鲤鱼开口苗的多不饱和脂肪酸、∑n-3+∑n-6和∑DHA+EP A含量较对照组显著升高(P<0.05),且2组之间差异显著(P<0.05);1,100 mg/L L-肉碱处理组鲤鱼开口苗的C/N比率较对照组和1 000 mg/L L-肉碱处理组显著降低(P<0.05)。【结论】本试验条件下,以质量浓度100 mg/L L-肉碱强化卤虫24 h后再投喂鲤鱼开口苗,可显著改善鲤鱼开口苗的脂肪酸组成和C/N比率。
【Objective】 Effects of L-carnitine enriched Artemia on fatty acids composition and C/N ratio in first feeding larvae of freshwater common carp（Cyprinus carpio） were studied to offer basis to improve culturing technology of the larvae.【Method】 The experimental larvae were reared in 50 L tank with stocking density of 40 fish/L at（25±1） ℃ for 21 days.The newly hatched Artemia nauplii,enriched with 4 levels（0,1,100,1 000 mg/L） of L-carnitine at（25±1） ℃for 12 h and 24 h respectively,were used as starter food for rearing larvae.【Result】 In the 12 h-enriched groups,∑C14-24 FA,SFA and MUFA contents were significantly（P0.05） lower by feeding 1 mg/L L-carnitine enriched nauplii than those of the control group;MUFA content was improved significantly（P0.05） by feeding 1 000 mg/L L-carnitine enriched nauplii than the control group,no significant differences（P0.05） were observed on PUFA,∑n-3＋∑n-6 and ∑DHA＋EPA content in the treatment groups;C/N ratio was decreased significantly（P0.05） by feeding 1 000 mg/L L-carnitine enriched nauplii than 1 mg/L L-carnitine enriching group and the control（P0.05）.In the 24 h-enriched groups,∑C14-24 FA and SFA contents were significantly（P0.05） lower in 1 and 100 mg/L L-carnitine enriching groups than those of the control group,but no significant differences（P0.05） were observed between them;∑C14-24 FA and SFA contents were improved significantly（P0.05） by feeding 1 000 mg/L L-carnitine enriched nauplii than the control group;MUFA content of larvae was decreased significantly（P0.05） by feeding 1 mg/L L-carnitine enriched nauplii than that of the other groups;no significant differences（P0.05） were observed on PUFA,∑n-3＋∑n-6 and ∑DHA＋EPA content between 1 mg/L L-carnitine enriching groups and the control;There was significantly（P0.05） higher PUFA,∑n-3＋∑n-6 and ∑DHA＋EPA content for larvae in 100 and 1 000 mg/L L-carnitine enriching groups than in the control,but significant differences
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2011年第6期31-36,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(30671621)
关键词
L-肉碱
强化卤虫
鲤鱼
开口苗
脂肪酸组成
C/N比率
L-carnitine
enriched Artemia
common carp（Cyprinus carpio）
first feeding larvae
fatty acids composition
C/N ratio
