摘要
提出了制备β－ 磷酸三钙（β－ ＴＣＰ） 粉末的新工艺，研究了多孔β－ ＴＣＰ 陶瓷（ 多孔β－ ＴＣＰ） 的成型和烧结条件。检测结果表明，该材料的Ｃａ／Ｐ 为１ ．５０ ，平均孔隙率为４２ ．３ ％ ，抗压强度为３４ ．８ ｋｇ／ｃｍ２ ，大孔孔径为４００ μｍ 左右，小孔孔径为５ μｍ左右，，在生理盐水中有微量溶解，溶血、急性毒性和致热源性均在安全范围内。制得骨形态发生蛋白（ＢＭＰ） 与多孔β－ ＴＣＰ 的复合人工骨（ＢＭＰ／ 多孔β－ ＴＣＰ） 。小鼠肌袋种植实验结果表明，该复合材料具有异位诱导成骨能力。兔桡骨缺损（１ ．５ ｃｍ） 的骨修复实验结果表明，ＢＭＰ／ 多孔β－ ＴＣＰ的修复效果明显优于多孔β－ ＴＣＰ 以及多孔羟基磷灰石陶瓷（ 多孔ＨＡ，或多孔ＨＡＰ） 。
The composite arificial bone of bone morphogenentic protein/porous β-tricalcium phosphate (BMP/p-β-TCP) with ability of osteoinducition and biodegradation has been studied.A new process preparing β-tricalcium phosphate (β-TCP) bioceramic powders has been developed.The problem that very diffcult filtering of gelatinous precipitate has been solved.The processing parameters in moulding and sintering were investigated.The results show that Ca/P ratio of the prepared p-β-TCP bioceramic is 1.50,porosity volume is 42.3%,diameter of macropores is 400 μm,diameter of micropores is 5μm,compressive strength is 34.8 kg/cm 2.The degredation experiment in vitro showed that it could be able to solve slightly in hormalsaline.The results of all tests of hemolysis,acute toxicity and pyrogen were found within normal range.The results of intramuscular implatation test showed that BMP/p-β-TCP has good osteoinduction.The BHP/p-β-TCP materials were implanted into 1.5cm radial defects of New Zealand rabbits,it has been showed that that bone repairing ability of BMP/p-β-TCP is superior to the orthes (p-β-TCP and P-HA).The BMP/p-β-TCP is suitable fo~ substitution of autogsaft bone on repairing spongy bone defectw.
关键词
Β-磷酸三钙
骨形态发生蛋白
生物陶瓷
人工骨
beta tricalcium phosphate,bone morphogenetic,protein,bioceramic porous structure,artificial bone
