摘要
以玉簪(Hosta plantaginea Aschers)为试材,采用石蜡切片法研究其大小孢子的发生及雌雄配子体的发育,比较了花蕾长度与雌雄配子体发育的对应关系,为进一步研究重瓣玉簪的形成原因和分子机制奠定胚胎学基础。结果表明:玉簪胚囊发育为贝母型;花药为四室,腺质绒毡层;花粉母细胞减数分裂为连续型;四分体呈四面体型;成熟花粉为三细胞结构,有2个较小的精细胞和1个较大的营养细胞。
Paraffin sectioning and microscopic technique are used to reveal the mega-/micro-sporogenesis and female-/male-gametogenesis of Hosta plantaginea Aschers. The relationship between the developmental stage of pistil and stamen and the size of bud were analysed to further study the molecular mechanisms of the double flower. The results showed that the form of host megaspore development was fritillaria typed embryonic. The anthers of the fertile line had four microsporangiates and the tapetum was belonged to the glandular type. Consecutive cytokinesis in microspore mother cell accompanies meiosis to produce tetrahedral tetrad. The mature pollen is 3-cellular type with two small sperms and one big vegetative cell.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2011年第3期295-299,共5页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(30901141)
关键词
玉簪
大小孢子发生
雌雄配子体发育
石蜡切片法
Hosta plantaginea Aschers
mega-/micro-sporogenesis
female-/male-gametophyte paraffin sectioning
