摘要
目的:研究中国汉族人群T淋巴细胞免疫球蛋白黏蛋白-3(Tim-3)基因标签单核苷酸多态性(tagSNP)rs11741184C/G和rs13170556A/G位点多态性与乙型肝炎病毒(HBV)感染转归的关系.方法:采用SNaPshot技术检测996例慢性乙型肝炎患者及301例急性HBV感染自限性恢复患者Tim-3基因rs11741184和rs13170556tagSNP位点的多态性,计算其基因型和等位基因分布频率及单体型分布频率.结果:Tim-3基因tagSNPrs11741184位点基因型CC、CG、GG在急性乙型肝炎患者中的分布频率分别为84.39%(254/301)、15.28%(46/301)、0.33%(1/301),在慢性乙型肝炎组中分布频率分别为86.04%(857/996)、13.65%(136/996)、0.3%(3/996),两组比较无统计学差异;Tim-3基因tagSNP rs13170556基因型AA、GA、GG在急性乙型肝炎患者中的分布频率分别为68.77%(207/301)、28.57%(86/301)、2.66%(8/301),慢性乙型肝炎组患者中的分布频率分别为68.07%(678/996)、28.41%(283/996)、3.51%(35/996),两组比较无统计学差异.单体型分析显示,中国汉族人群中存在3种单体型(C-A、C-G、G-A),这3种单体型在急性乙型肝炎组分别为75.08%、16.94%、7.97%,慢性乙型肝炎组分别为75.15%、17.72%、7.13%,3种单体型与HBV感染的转归均无相关性.结论:中国汉族人群中Tim-3基因tagSNPrs11741184和rs13170556位点多态性可能与HBV感染的转归不具相关性.
AIM：To investigate the relationship between single nucleotide polymorphisms（SNPs）of the T cell immunoglobulin musin-3（Tim-3）gene and outcome of hepatitis B virus（HBV）infection in a Chinese Han population.METHODS：Two tagSNPs of the Tim-3 gene（rs11741184 and rs13170556）were genotyped,using the SNaPshot method in 996 patients with chronic HBV infection group and 301 patients with acute self-limiting HBV infection.The genotypes,allele frequencies and haplotypes of the two Tim-3 tagSNPs were compared between the two groups of patients.RESULTS：The frequencies of CC,CG and GG genotypes at the rs11741184 locus were 84.39%（254/301）,15.28%（46/301）and 0.33%（1/301）in patients with acute selflimiting HBV infection,and 86.04%（857/996）,13.65%（136/996）and 0.3%（3/996）in patients with chronic HBV infection,respectively.There were no statistical differences in the genotype frequencies at the rs11741184 locus between the two groups of patients（all P0.05）.The frequencies of AA,GA and GG genotypes at the rs13170556 locus were 68.77%（207/301）,28.57%（6/301）and 2.66%（8/301）in patients with acute selflimiting HBV infection,and 68.07%（678/996）,28.41%（283/996）and 3.51%（35/996）in patients with chronic HBV infection,respectively.There were also no statistical differences in the genotype frequencies at the rs13170556 locus between the two groups of patients（all P0.05）.Three haplotypes for Tim-3 tagSNPs（C-A,C-G,G-A）were found in the Chinese Han population,and their haplotype frequencies were similar between patients with acute selflimiting HBV infection（75.08%,16.94%,7.97%）and those with chronic HBV infection（75.08%vs 75.15%,16.94%vs 17.72%,7.97%vs 7.13%,all P0.05）.CONCLUSION：The two Tim-3 tagSNPs may not be associated with outcome of HBV infection in Chinese Han population.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第14期1506-1510,共5页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.81072342
973前期专项课题基金资助项目
No.2009CB526411~~
