摘要
在PAHs污染土壤生物修复过程中,以黑麦草、苜蓿为修复植物,固定化微生物菌剂为外源微生物,通过盆栽实验研究了不同处理对土壤酶活性的影响,以及酶活性与PAHs的去除效果之间的相关性。结果表明,植物修复、微生物修复及两者联合修复均显著提高了土壤PAHs的去除效果,其中黑麦草和苜蓿与微生物菌剂联合修复效果分别达到37.57%和38.41%,比单独的植物修复和菌剂修复高出一倍左右。各种生物修复同时促进了土壤多酚氧化酶、脱氢酶及脲酶的活性,减少了过氧化氢酶活性。过氧化氢酶活性与多酚氧化酶活性呈显著负相关(P<0.05)、而脲酶与多酚氧化酶显著正相关(P<0.05)。进一步分析表明PAHs的去除率与脱氢酶活性极显著正相关(P<0.01),与多酚氧化酶活性呈显著正相关(P<0.05),与过氧化氢酶活性呈负相关(P=0.564);因此,在PAHs污染土壤生物修复过程中,可以选择土壤脱氢酶活性和多酚氧化酶活性作为PAHs修复效率的微生态指示指标。
Polycyclic aromatic hydrocarbons（PAHs） are an important group of organic micropollutants（xenobiotics） which are widely distributed in the environment.This study aimed to compare the PAHs degradation effects by bioremediation using immobilized microbes,phytoremediation using ryegrass（Lolium perenne L.） and alfalfa（Medicago sativ L.）,and combination of the above two approaches on the PAHs contaminated soil.Attention was paid on variation of the soil enzyme activity,as well as correlation between the PAHs removal rate and the enzyme activity during the bioremediation process.The experimental results showed that although phytoremediation,microbial remediation and integrated plant-microorganism bioremediation would all degrade PAHs effectively,the PAHs removal by ryegrass-（immobilized） microbe and alfalfa-（immobilized） microbe would reach up to 37.57% and 38.41%,respectively,which were 100% higher than either phytoremediation or microbial remediation method.It was found that bioremediation/phytoremediation of PAHs contaminated soil would increase the soil enzyme activity of dehydrogenase,polyphenol oxidase,urease,and decrease the enzyme activity of catalase.In particular,rhizosphere soil dehydrogenase and polyphenol oxidase activities were significantly correlated to concentration of PAHs.They might thus be taken as degradation indicators of soil PAHs during bioremediation process.
出处
《生态环境学报》
CSCD
北大核心
2011年第4期691-695,共5页
Ecology and Environmental Sciences
基金
国家十二五科技支撑计划项目(2011BAJ06B02)
辽宁省高等学校优秀人才支持计划(LR201028)
国家自然科学基金项目(50908151)
沈阳市科技计划(F10-221-4-00)
关键词
PAHS
生物修复
土壤酶活性
脱氢酶
多酚氧化酶
PAHs
bioremediation
phytoremediation
soil enzyme activity
dehydrogenase
polyphenol oxidase
