摘要
目的探讨孕酮对K562和NB4白血病细胞系的抑制增殖和诱导分化作用。方法采用细胞计数,XTT实验观察孕酮对白血病细胞增殖能力的影响,采用Wright-Giemsa染色,联苯胺染色,NBT还原实验和流式细胞术观察孕酮对白血病细胞的诱导分化作用。结果不同浓度孕酮连续作用白血病细胞5天,液体培养显示细胞数明显减少,第5天处理组的XTT值显著低于对照组,并呈剂量依赖关系;液体培养和XTT均显示孕酮对NB4细胞的增殖并无显著影响。K562细胞联苯胺染色OD值显著升高,与对照组比较差异有统计学意义(P<0.01),NB4细胞NBT还原能力提高(P<0.01),并呈剂量依赖性。形态学观察孕酮可诱导K562细胞和NB4细胞趋向成熟分化,流式细胞术检测孕酮处理后K562和NB4细胞膜CD71和CD11b分化抗原表达阳性率分别为85.72%和61.28%。结论孕酮可显著抑制K562白血病细胞增殖,诱导K562和NB4白血病细胞向成熟分化。
Objective To investigate the antiproliferation and differentiation-inducing effects of progesterone on K562 and NB4 leukemia cell line.Methods The antiproliferative effects of progesterone on leukemia cells were studied by cell count and XTT assay.The differentiation was analyzed by morphology,benzidine stain,NBT assay and flow cytometry.Results The growth of K562 cells was inhibited after treatment with progesterone for 5 days in cell count and XTT assay.But the NB4 cells inhibition rates in cell count and XTT assay was not significantly different after treatment with progesterone for 5 days.The morphology obxervation showed the differentiation tendency of K562 and NB4 cells treated with progesterone.It was also verified by bezindine stain that progesterone also induced K562 cells toward erythroid differentiation.In NBT assay,NB4 cells showed the tendency toward myelocyte differentiation.Data from flow cytometry showed that after treatment with 10-5mol/L progesterone for 4 days,the percentage of positive CD71 expression in differentiated K562 cells and positive CD11b expression in differentiated NB4 cells was 85.72% and 61.28%.Conclusion Progesterone could inhibit the proliferation of K562 cell lines and induce K562 and NB4 cells toward maturation differentiation.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2011年第6期632-635,共4页
Cancer Research on Prevention and Treatment
基金
河南省教育厅自然科学研究计划资助项目(2010B330002
2010B320011)
