摘要
目的:筛选具有人参皂苷转化能力的真菌菌株,用于生产稀有人参皂苷compound K。方法:通过液态发酵,以三七茎叶总皂苷为底物,采用TLC和HPLC-ELSD检测转化产物,从12株植物病原真菌中筛选能够转化产生人参皂苷compoundK的最佳菌株。结果:获得一株高效人参皂苷转化真菌,可以转化产生人参皂苷compound K,该菌株为镰刀属真菌串珠镰孢Fusarium moniliforme。结论:串珠镰孢对三七茎叶总皂苷具有较高的转化效率,有望用于生产制备compound K等低含量高活性的稀有人参皂苷。
Objective： To screen a new strain which can transform panaxadiol saponins into the rare ginsenoside compound K.Method： The total saponins in stems and leaves of Panax notoginseng was used as a substrate in the liquid state fermentation process,and the results were detected by TLC and HPLC-ELSD to screen a strain from twelve plant pathogenic fungi which can produce ginsenoside compound K.Result： Fusarium moniliforme was found to transform the total saponins to ginsenoside compound K efficiently in the all twelve fungal strains.In the fermentation process,ginsenoside Rb1 was transformed almost completely,and the content of ginsenoside Rd was decreasing evidently.Conclusion： F.moniliforme is selected as a new high-yield strain.It is expected to be used to produce the high activity infrequent ginsenoside compound K and to improve the content of active principles in medicinal plants.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2011年第12期1596-1598,共3页
China Journal of Chinese Materia Medica
基金
国家科技支撑计划项目(2007BAI38B03)
