摘要
目的:观察神经肽Y对3T3L1前脂肪细胞增殖和分化的影响,并对其机制进行初步探讨。方法:3T3-L1细胞由3-异丁基-1-甲基黄嘌呤(3-isobutyl-1-methylxanthine,IB-MX)、胰岛素和地塞米松联合诱导分化,2 d后将细胞分为空白对照组(未加任何诱导剂组)、经典诱导组(胰岛素诱导)和NPY干预组(10-8、10-9、10-10mol/L NPY)。培养的第7、12天用相差显微镜观察各组细胞形态的变化,培养第12天用油红O染色观察脂肪细胞分化程度。MTT检测细胞增殖。Western blot检测脂肪细胞分化相关基因过氧化物体增殖剂活化受体-γ(peroxisome proliferator-activated receptor gamma,PPAR-γ)、CAAT/增强子结合蛋白-α(CCAAT/enhancer-bind-ing protein-α,C/EBP-α)蛋白的表达。结果:10-8mol/L NPY能促进3T3-L1细胞的分化。10-9mol/L NPY,10-8mol/L NPY均能促进3T3-L1细胞增殖。10-8mol/L NPY增加C/EBPα、PPARγ的表达。结论:NPY促进3T3L1前脂肪细胞的增殖和分化,其机制可能与上调PPARγ、C/EBPα的表达有关。
AIM： To observe the effect of neuropeptide Y on the proliferation and differentiation of 3T3L1 preadipocytes and detect the mechanics.METHODS： 3T3-L1 preadipocytes were induced to differentiation by the cocktail medium containing 3-isobutyl-1-methylxanthine（IBMX）,dexamethasone and insulin.After 2 days,the cells were divided into 3 groups： the control group（without inducer）,the routine group（insulin used as inducer） and the experiment group（10-8,10-9,10-10 mol/L NPY used as inducer）.At day 7 and 12,cell differentiation was observed by phase contrast microscope.At day 12,Oil red O staining was performed.Proliferation capacities of 3T3L1 cells were assessed by MTT.Western blot was used to know the protein level of peroxisome proliferator-activated receptor gamma（PPAR-γ） and The CCAAT/enhancer-binding protein-α（C/EBP-α）.RESULTS： NPY 10-8 mol/L promotes the differentiation of 3T3L1 preadipocytes,10-9 mol/L,10-8 mol/L NPY increased 3T3-L1 cells proliferation.The protein levels of PPAR-γ and C/EBP-α were up regulated by 10-8 mol/L NPY.CONCLUSION： Neuropeptide Y promotes the differentiation and Proliferation of 3T3-L1 cells,this effect may be related to up-regulation of PPAR-γ and C/EBP-α.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2011年第6期605-607,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
湖南省自然科学基金资助项目(10JJ5045)
