摘要
从60株乳酸菌中筛选出22株具有抗甜瓜枯萎病菌活性的乳酸菌菌株,其中9株具有较强抗甜瓜枯萎病菌活性.对这9株乳酸菌的抗植物致病真菌谱进行研究发现,9株菌对番茄早疫病菌(Alternaria solani)、甜瓜疫霉菌(Phytophthora drechsleri Tucker)、苹果炭疽病菌(Glomerella cingulate)、灰葡萄孢霉菌(Botrytis cinerea)的生长均有抑制作用.其中干酪乳杆菌IMAU10041对甜瓜枯萎病菌具有较强的抑制作用,并且对甜瓜疫霉和番茄早疫病菌有较高的抑菌活性.分别经过调节pH、加热、蛋白酶处理干酪乳杆菌IMAU10041的发酵滤液后,发现干酪乳杆菌IMAU10041发酵液具有一定的热稳定性;在pH 4.0时具有最大抑菌活性,在碱性条件下抑菌活性迅速失活;发酵液经胰蛋白酶、中性蛋白、蛋白酶K处理后失去部分抑菌活性,这表明干酪乳杆菌IMAU10041发酵液中可能含有一定的蛋白类抑菌物质.
Twenty-two of the 60 colonies of lactic acid bacteria isolated from the koumiss showed antifungal activity against Fusarium oxysporume,ven 9 colonies of these lactic acid bacteria was better.The broad spectrum of antifungal activity of the 9 colonies was indicated that the 9 colonies could inhibit Alternaria solani,Phytophthora drechsleri Tucker,Glomerella cingulata and Botrytis cinerea.Lactobacillus casei IMAU10041 have more stronger inhibitory action to the F.,oxysporum.The effect of pHt,emperature and protease on the antifungal activity of L.casei IMAU10041 was determined.The activity substance was heat stable.The maximum antifungal activity was observed at pH 4.0,with the increase of pHt,he antifungal activity was lost rapidly.After enzymatic treatment of supernatant with trypsin,neutral protease and proteinase Kl,oss of part of the antifungal activity was observed which indicated there are other proteinaceous substance with the antifungal activity.
出处
《天津科技大学学报》
CAS
2011年第3期12-15,23,共5页
Journal of Tianjin University of Science & Technology
基金
国家自然科学基金资助项目(2087611630900961)
乳品生物技术与工程教育部重点实验室开放课题
关键词
植物致病真菌
抗真菌活性
甜瓜枯萎病菌
乳酸菌
干酪乳杆菌
plant pathogenic fungi
antifungal activity
Fusarium oxysporum
lactic acid bacterium
Lactobacillus casei
