摘要
为优化核桃试管苗生长条件,向核桃优良品种规模化栽培提供大量优良的种植材料,以‘香玲’核桃实生苗为材料,对基本培养基的类型(MS,DKW,WPM)和凝固剂种类(琼脂,Phytagel,二者结合)进行了筛选,并采用完全随机试验设计研究了细胞分裂素种类和浓度对其试管苗生长的影响.试验结果表明:基本培养基与凝固剂的种类,以及细胞分裂素的种类与浓度对芽苗生长的影响很大.以DKW为基本培养基,2.2 g/L Phytagel为凝固剂,激素配比为0.8 mg/L BA+0.01 mg/L IBA,有利于芽苗增殖,增殖可达4.70;后在0.8 mg/L KT+0.01 mg/L IBA进行壮苗,即可进行生根培养.不同基因型对基本培养基中无机盐成分的需求不同,含有较丰富矿物质的Phytagel有利于芽苗生长.BA促进腋芽的萌动和增殖,而KT则有利于壮苗.
To provide abundant and excellent colonial plant materials,the optimum growth conditions of in vitro walnut shoot were studied.Using the seedlings of walnut ＇Xiangling＇ as materials,the types of nutrient media（MS,DKW and WPM） and gelling agents（Difco Bacto agar,Phytagel and a mixture of Phytagel and Difco Bacto agar） were screened,and the effects of different types and concentrations of cytokinin on growth of the seedlings were studied with randomized complete-block design.The results indicated that different types of nutrient media and gelling agents had great effects on growth of the seedlings as well as the types and concentrations of cytokinin.DKW basic medium supplemented with 0.8 mg/L BA ＋ 0.01 mg/L IBA and solidified with 2.2 g/L Phytagel,favoured proliferation of the shoots,and the number of proliferation was 4.70.After cultured in present of 0.8 mg/L KT ＋ 0.01 mg/L IBA for strong stock,the shoots could be rooted.Different genotypes need basic media with different compositions of inorganic salt.Phytagel containing rich minerals is helpful for bud and shoot growth.BA promotes axillary to germinate and proliferate,while KT is helpful for shoot elongation.Fig 4,Tab 3,Ref 22
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2011年第3期359-363,共5页
Chinese Journal of Applied and Environmental Biology
基金
河南省科技厅重大科技攻关项目(No.092101110600)资助~~
关键词
'香玲’核桃
试管苗
基本培养基
凝固剂
细胞分裂素
增殖
伸长
walnut ＇Xiangling＇
in vitro shoot
basic medium
gelling agent
cytokinin
proliferation
elongation
