摘要
利用PCR-DGGE技术对天津新港潮间带（东经117°45′11.4″~45′12.4″,北纬39°2′42.8″~3′14.6″）沉积物中的细菌群落组成和优势菌群进行了调查,通过Quantity One软件分析不同样品的DGGE图谱,发现9个站位的细菌种类、数量和多样性都存在明显差别,表明该潮间带细菌群落的空间异质性较高.细菌16S rDNA V3区特征片段经DGGE分离、条带切割,克隆、测序后,进行BLAST比对和系统进化分析,结果表明天津新港潮间带沉积物中主要优势菌群归属于未培养的变形菌门（Proteobacteria）和其它一些环境样品中的未归类菌群的细菌克隆;同时还发现许多条带序列与来自受不同污染的环境样品中的未培养细菌克隆具有较高的同源性（94%~100%）,提示该区域受到不同类型污染物（如硫化物、重金属以及芳烃类污染物）的污染.
The bacterial community structure and predominant bacterial species were investigated by PCR-Denaturing Gradient Gel Electrophoresis（DGGE） in intertidal sediment in Xingang Area（E 117°45′11.4″~45′12.4″,N 39°2′42.8″~3′14.6″）,Tianjin,China.The DGGE profile was analyzed by Quantity One Software,and the results showed that there were obvious differences in bacterial species,number and population diversity among the 9 sediment samples,which indicated high spatial heterogeneity of bacterial community in this area.Twenty DGGE bands reflecting predominant phylotypes were excised,cloned,sequenced and BLAST.The results showed that the bacterial phylotypes were composed of uncultured Proteobacteria and other unclassified bacterial clones from environmental samples.16S rDNA sequences of some bands had high homology（94%~100%） with the uncultured bacterial clones isolated from different polluted environment samples,which suggested the studied area polluted by various contaminants（e.g.sulfide,heavy metal,aromatic hydrocarbon）.Fig 2,Tab 3,Ref 12
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2011年第3期303-306,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家基础研究发展规划项目(“973”项目)(No.2007CB407306)资助
Supported by the National Basic Research & Development Program of China(“973”Program,No.2007CB407306)
