摘要
依据慢性粒细胞白血病BCR/ABL融合基因的碱基序列,设计了一种新型发夹结构锁核酸(locked nu-cleic acids,LNA)探针,将该探针借助Au—S键固定在金电极表面构建了特异的生物传感器.LNA探针与目标链DNA杂交,以本实验室合成的苯甲酸二聚铜配合物([Cu2(C7H5O2)4(C2H6O)2],简称[Cu(R)2]2+)为杂交指示剂,应用差示脉冲伏安法进行检测,表现出良好的响应信号.该新型锁核酸传感器能较好的区分完全互补链DNA、单碱基错配链DNA.互补链DNA检测的线性范围为1.0×10-8～1.0×10-6 mol.L-1,检出限2.0×10-9mol.L-1.
In this article,a new type of structure hairpin locked nucleic acids（LNA） probe was designed to detect BCR/ABL fusion gene in Chronic Myelocytic Leukemia.The LNA probe was immobilized on the gold electrode（AuE） through sulfur-Au interaction to construct specific electrochemical biosensor.The electrochemical response of the sensor to hybridization of the LNA probe with the target DNA was studied using [Cu（R）2]2＋ as an electrochemical indicator.The optimal condition was discussed.The experimental results indicated that in pH 7.4 PBS buffer solution,this new method has excellent selectivity for single base mismatch and complementary after hybridization.The linear relationship between the increased oxidation peak current of [Cu（R）2]2＋ and the concentration of complementary strand was observed in the range of 1.0×10-8~1.0×10-6 mol/L.The detection limit was 2.0×10-9 mol/L.
出处
《电化学》
CAS
CSCD
北大核心
2011年第2期180-185,共6页
Journal of Electrochemistry
基金
国家自然科学基金项目(20675015)
国家863计划(No.2008AA02Z433)
福建省自然科学基金(No.2010J06011
2010J01032)
福建省教育厅(No.JA10155)
福建省高校产学研科技重点项目(2010Y4003)资助
