摘要
目的:建立稳定的小鼠异位小肠移植模型。方法:应用C57BL/6小鼠进行同基因异位小肠移植。手术采用非造口方法,将移植肠近端结扎,远端与自体回肠末段吻合。结果:连续进行小鼠异位小肠移植手术33只,手术完成率为97%,1只小鼠术中血管吻合失败,2只小鼠术后因不可逆的低血容量性休克死亡,移植总成功率为90.9%。结论:采用非小肠造口方法建立的小鼠异位小肠移植模型成功率高,为小肠移植的基础研究提供了理想的工具。
Objective： To establish murine model of heterotopic small bowel transplantation（SBT）.Methods： Syngeneic heterotopic SBT was performed between C57BL/6 mice.According to stoma-free strategy,the proximal end of the graft was closed and the distal end was anastomosed with the nave terminal ileum.Results： Heterotopic SBT was performed in 33 cases consecutively.Only one operation was failed because of intra-operative bleeding,and total accomplishing rate of operation was 97%（32/33）;two cases were died of irreversible hypovolemic shock postoperatively,and overall success rate was 90.9%（30/33）.Conclusion： The stoma-free heterotopic SBT in mice is of high success rate,and it is an ideal tool for further research of SBT.
出处
《肠外与肠内营养》
CAS
北大核心
2011年第3期176-178,共3页
Parenteral & Enteral Nutrition
基金
江苏省科技攀登计划(BK2008034)
关键词
小鼠
异位小肠移植
动物模型
Mice
Heteropotic small bowel transplantation
Animal model
