摘要
目的观察先天性心脏病(CHD)并发肺动脉高压(PH)患者肺组织中内皮型一氧化氮合酶(eNOS)与基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶组织抑制因子-1(TIMP-1)的表达变化,并探讨其意义。方法光镜下观察60例CHD患者肺小动脉形态,按照Heath-Edwards PH分级法分组,CHD无PH患者14例为对照组,CHD合并PHⅠ、Ⅱ、Ⅲ级46例为观察组。采用RT-PCR技术检测患者肺组织中eNOS mRNA、MMP-1 mRNA、TIMP-1 mRNA。结果 MMP-1 mRNA、TIMP-1 mRNA、TIMP-1 mRNA/MMP-1 mRNA在观察组的表达高于对照组(P均<0.01),eNOS mRNA则低于观察组(P<0.01)。MMP-1 mRNA与TIMP-1 mRNA的表达呈正相关(r=0.879,P<0.01);MMP-1 mRNA、TIMP-1 mRNA、TIMP-1 mRNA/MMP-1 mRNA与PH分级呈正相关(r分别为0.858、0.802、0.315,P均<0.05),eNOS mRNA与PH分级呈负相关(r=-0.714,P<0.01);TIMP-1 mRNA、MMP-1 mRNA、TIMP-1mRNA/MMP-1 mRNA与eNOS mRNA呈负相关(r分别为-0.607、-0.642、-0.777,P均<0.01)。结论 CHD合并PH患者肺组织中eNOS、MMP-1、TIMP-1表达失调,参与肺血管重构及PH形成过程。
Objective To study the change and significance of eNOS,MMP-1 and TIMP-1 expression in lung tissue of patients with congenital heart disease（CHD）and pulmonary hypertension（PH）.Methods CHD patients were divided into different groups according to Heath-Edwards pulmonary vessel pathologic grading： control group（non-PH）14 cases,observation group（PHⅠ,PHⅡ,PH Ⅲ）46 cases.The eNOS mRNA,MMP-1 mRNA and TIMP-1 mRNA were detected by RT-PCR in the lung tissue of these patients.Results The levels of MMP-1 mRNA,TIMP-1 mRNA and TIMP-1 mRNA/MMP-1 mRNA were raised in observation group with control group（P0.01）,eNOS mRNA was decreased in observation group with control group（P0.01）.There was significant positive correlation between the MMP-1 mRNA and TIMP-1 mRNA（r=0.879,P0.01）.There were significant positive correlation between MMP-1 mRNA,TIMP-1 mRNA,TIMP-1 mRNA/MMP-1 mRNA and the grade of PH（r were 0.858,0.802 and 0.315,all P0.05）.There was significanthy negative correlation between the eNOS mRNA and the grade of PH（r=-0.714,P0.01）.There were singificantly negative correlation between TIMP-1 mRNA,MMP-1 mRNA,TIMP-1 mRNA/MMP-1 mRNA and eNOS mRNA（r were-0.607,-0.642 and-0.777,all P0.01）.Conclusion eNOS and MMP-1/TIMP-1 imbalance may contribute to pulmonary vascular remodeling in CHD with PH.
出处
《山东医药》
CAS
北大核心
2011年第18期10-11,共2页
Shandong Medical Journal
基金
广西壮族自治区自然科学基金资助项目(0640087)
关键词
肺动脉高压
先天性心脏病
一氧化氮合酶
基质金属蛋白酶
基质金属蛋白酶组织抑制因子
pulmonary hypertension
congenital heart disease
nitric oxide synthase
matrix metalloproteinases
tissue inhibitor of matrix metalloproteinases
