摘要
目的:对不同提取方法所得的卷柏提取物进行雌激素活性筛选,探索卷柏发挥雌激素作用的活性部位。方法:采用小鼠子宫增重试验,将卷柏分别以70%乙醇回流所得总部位经聚酰胺柱分离;95%乙醇回流药渣经水提取;药渣水提物去除多糖等所得的卷柏各组分进行雌激素活性筛选。运用HPLC指纹图谱技术对卷柏95%醇提物、药渣水提物进行分析。结果:70%乙醇回流所得的卷柏总部位、药渣水提物高剂量、药渣水提物去多糖组与空白组比较,均能显著性增加小鼠子宫系数(P<0.01),卷柏其余各组与空白组相比无明显作用;HPLC指纹图谱显示卷柏95%醇提物中穗花杉双黄酮为其主要成分,而药渣水提物中基本不含穗花杉双黄酮。结论:95%乙醇回流药渣水提法与聚酰胺柱分离法相比,前者更适合于卷柏雌激素活性物质的提取分离;水溶性除多糖物质为卷柏中发挥雌激素样作用的有效部位;HPLC指纹图谱结果提示,穗花杉双黄酮不是卷柏发挥雌激素作用的成分。
Objective：To identify the suitable extraction method and polarity for estrogenic active substance in Selaginella tamariscina,through the estrogenic active screening of different extracts of S.tamariscina from different extraction methods.Method：Selaginella tamariscina was extracted by 70% ethanol to obtain the total fraction.The total fraction was separated by polyamide column to obtain the water fraction,50% ethanol and 80% ethanol fractions;S.tamariscina was refluxed by 95% ethanol to obtain the 95% ethanol extract,and the residue was decocted by water to obtain the residue water extract;the residue water extract was removed polysaccharides to obtain non-polysaccharides part of water-soluble components.Then the uterine weight method in mice was adopted to detect the estrogenic activity of all these different extracts.The 95% ethanol extract and the residue of water extract were analyzed by high-performance liquid chromatography（HPLC） fingerprint.Result：Compared with the control group,the total fraction,high dose of the water extract residue and non-polysaccharides part of water-soluble components group significantly increased uterus coefficient（P0.01）,while the other groups of S.tamariscina showed no significantly effects（P0.05）;HPLC fingerprint demonstrated that amentoflavone was the main component of the 95% ethanol extract,but no amentoflavone was observed in the residue water extract.Conclusion：The extraction method of 95% ethanol refluxing and the residue water decocting was more suitable for extracting the estrogenic active substance of S.tamariscina;the non-polysaccharides part of water-soluble components played an important role in estrogen activity in S.tamariscina;HPLC fingerprint suggested that amentoflavone was not the estrogenic active component of S.tamariscina.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第12期1-5,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金(81073034)
国家"重大新药创制"科技重大专项(2009ZX09103440)
河南省重大公益性科研招标项目(08110091800)
关键词
卷柏
雌激素类活性成分
子宫系数
提取方法
HPLC指纹图谱
Selaginella tamariscina
estrogenic active substance
uterus coefficient
extraction
HPLC fingerprint
