摘要
目的:探讨支气管哮喘小鼠急性外周炎症反应和血单核细胞p38MAPK表达的变化及地塞米松(DEX)对其的影响。方法:采用蛋白质印迹和ELISA方法检测PBMCs核蛋白p38MAPK的表达及细胞上清液中IL-4、IL-5的蛋白质浓度。结果:哮喘组PBMCs核蛋白p38MAPK表达及细胞上清液中IL-1、IL-6的蛋白质浓度较正常对照组显著升高(P<0.01),DEX处理组核蛋白p38MAPK表达及细胞上清液中IL-1、IL-6的蛋白质浓度较哮喘对照组显著降低(P<0.01)。通过直线相关分析发现,PBMCs核蛋白p38MAPK表达与培养上清液中IL-1、IL-6蛋白质含量之间均呈显著正相关(r分别=0.72、0.56,P<0.01)。结论:地塞米松可在一定程度上减轻哮喘小鼠的气道炎症反应,作用机制可能是通过下调外周血单核细胞p38MAPK过度表达,从而抑制依赖于p38MAPK信号转到通路的急性气道炎症反应。
Objective： To study the changes ofp38 MAPKin peripheral blood mononuclear cells（PBMCs）in rats with bronchial asthma and the elects of dexamethasone （DEX）on it. Methods： PBMCs were collected from asthmatic rats and normal rats. Using Western blot and ELISA, the expression of phosphor-p38 MAPK in PBMCs and the concentration of IL-1, IL-6 in supematants were respectively measured. Results： The expression of phosphor-p38 MAPK in PBMCs and the concentration of IL-1, IL-6 in supematants were higher in asthmatic group than those in normal group P〈0.01）. By DEX treated, the above indexes were decreased significantly compared with those in asthmatic group（P〈0.01）. There were positive correlations between the expression of phosphor-p38 MAPK an d the concentration of IL-1, IL-6 in supematants （r=0.72,0.56, P〈0.01）. Conclusion：p38 MAPK may play a role in pathophysiological process ofasthma. DEX could effectively treat asthma by inhibiting the expression ofp38 MAPK.
出处
《中国伤残医学》
2011年第6期23-24,共2页
Chinese Journal of Trauma and Disability Medicine
