摘要
目的 探讨细胞凋亡抑制基因ｂｃｌ－ ２ 和凋亡促进基因ｂａｘ 在胆管癌组织中的表达。方法 应用免疫组化方法对２０ 例胆管癌（ＣＨＣ）和７例先天性胆总管囊肿（ＣＣＣ）组织中ｂｃｌ－ ２和ｂａｘ 蛋白进行检测。结果 ２０例ＣＨＣ中有１例ｂｃｌ－ ２ 蛋白表达阳性（５％ ），７例ＣＣＣ中ｂｃｌ－ ２均表达阴性。２０例ＣＨＣ中有１１ 例ｂａｘ 蛋白表达阳性（５５％ ），７例ＣＣＣ中仅１ 例表达阳性（１４．２９％ ），两组间差异有显著意义（Ｐ＜ ０．０１）。结论 ｂｃｌ－ ２ 和ｂａｘ 蛋白的改变在胆管癌发生发展过程中不起重要作用。
Objective To inverstigate the role of bcl-2 (an apoptosis suppressing oncogene)and bax (an apoptosis accelerating oncogene)in the development of cholangiocarcinoma.Method We investigated bcl-2 and bax expression by means of immunohisto chemical chemical technique in 20 cases of cholangiocarcinoma and 7 cases of congenital choledochal cyst.Results Bcl-2 was positive in 5% of cholangiocarcinoma and all congenital choledochalcyst tissues were negative.Bax was positive in 55% of cholangiocarcinoma and in 14.29% of congenital choledochal cyst tissue.Bax staining intensity was significantly weak in congenital choledochal cyst than that in cholangiocarcinoma tissue.Conclusion These results suggested that the changes of expression of bcl-2 and expression of bax in cholangiocarcinoma does not play an important role in the development of cholangiocarcinoma.
出处
《肝胆外科杂志》
1999年第5期397-398,共2页
Journal of Hepatobiliary Surgery
关键词
胆管癌
癌基因蛋白类
基因表达
细胞凋亡
Cholangiocarcinoma Oncogene protein Gene expression Apoptosis
