摘要
[Objective] This study was to explore the subcellular localization of aquaporins OsPIP2-6 in rice. [Method] A key rice aquaporins gene OsPIP2-6 was cloned and used for construction of a transient expression vector,which was then transformed into onion epidermis via particle bombardment for confocal microscopy analysis using YFP gene as a reporter gene. [Result] The results showed that rice aquaporins OsPIP2-6 was mainly located in the plasma membrane. [Conclusion] Our results provided theoretical basis for further understanding plant aquaporins.
[目的]探讨水稻水通道蛋白OsPIP2-6的亚细胞定位。[方法]克隆了水稻中的重要通道蛋白OsPIP2-6基因,构建了瞬时表达载体,通过基因枪法在洋葱表皮中进行瞬时表达,并通过激光共聚焦显微镜进行了观察。[结果]水稻水通道蛋白OsPIP2-6主要定位在细胞质膜上,证明了OsPIP2-6是个水通道蛋白。[结论]为植物水通道蛋白的深入研究提供了理论依据。
基金
Supported by Program for Science and Technology Development in Shijiazhuang City(10120803)
Doctoral Research Fund of Shijiazhuang University(2007012)~~
