摘要
【目的】研究原位异种移植肝癌模型AFP基因启动子区异常甲基化状态的检出状况,探讨其在肝癌发病机制研究中的应用。【方法】采用甲基化特异性PCR(MSP)和亚硫酸氢盐测序法(BSP),以人成纤维细胞基因组甲基化状态为正常对照,对10例原位移植人肝癌裸鼠模型肝癌组织中AFP基因启动子区甲基化状态进行分析和定位。【结果】10例模型肝癌组织中AFP基因启动子区甲基化状态均发生了不同程度的异常。【结论】原位移植人肝癌模型的肝癌组织中可以检测到AFP基因启动子区的去甲基化变化,MSP和BSP作为简便而有效的技术组合可以应用于基因甲基化状态的鉴定中。
[Objective] To investigate the aberrant methylation status in the promoter region of AFP gene and try to find out it＇ s application in the research on the hepatocarcinogenesis mechanism. [ Methods] The methylation status of the AFP gene in the liver cancers from 10 orthotopic implanted nude mice models of human hepatocellnlar carcinoma were detected and analyzed by MSP and BSP. The ones of genome from human fibroblasts cells were the controls. [Results] The methylation status from all the mice had changed. [Conclusion ] The methylation status in the promoter region of AFP gene is abnormal. MSP and BSP are the efficient and essential methods.
出处
《武警医学院学报》
CAS
2011年第8期622-624,F0003,共4页
Acta Academiae Medicinae CPAPF
关键词
原位移植
AFP基因
甲基化
肝癌
Orthotopic implantation
AFP gene
Methylation
Hepatoma
