摘要
目的探讨间质性膀胱炎（IC）组织中Uroplakin（UP）基因家族表达的临床意义。方法采用RTPCR方法检测29例IC患者膀胱黏膜组织标本中UPⅠa、Ⅰb、Ⅱ、Ⅲ以及Ⅲ-δ4mRNA（UPⅢ选择性黏接变异体）的表达，免疫组化染色法检测UPⅢ蛋白表达。16例正常膀胱黏膜组织标本作为对照组。结果IC患者膀胱黏膜组织中UPⅢ-δ4 mRNA表达中位值为4．80，对照组为0．22，组间差异有统计学意义（P〈0．001）；无Hunner溃疡患者膀胱黏膜组织中UPⅠa、Ⅰb、Ⅱ、Ⅲ以及Ⅲ-δ4mRNA基因表达值分别为4．99、3．31、2．75、15．38、11．48，显著高于有溃疡组的0．04、0．29、0．09、0．11、0．34，组间差异有统计学意义（P％0．01）。有溃疡组UPⅠa、Ⅰb、Ⅱ、ⅢmRNA表达值均显著低于正常对照组的3．22、4．22、3．06、0．95，组间差异有统计学意义（P〈0．01）；无溃疡组中UPⅢ与UPⅢ-34mRNA表达水平显著高于对照组，表达中位值分别为对照组的5．6倍（15．38与2．76）和26．5倍（11．48与0．43）。UP基因表达与其他临床指标无显著相关。结论IC膀胱黏膜组织中UP基因家族表达发牛异常改变，UPⅢ-δ4mRNA有可能成为非溃疡型IC的特异性标志物。
Objective To detect gene expressions of Uroplakin （UP） family in interstitial cystitis （IC）. Methods Gene expression of UP Ia, Ib, II, III, and III-δ4 was quantitatively measured in bladder biopsy samples from patients with IC （n--29） and control subjects （n= 16） using real-time RT PCR. Results UP III-delat4, a splicing variant of UP III, was significantly up-regulated to 4.80 in IC samples （P〈0. 001）. When IC patients were divided into two groups, one with and one without ulcerative changes, the UP Ia, UP Ib, UP II, UP III and III-c34 genes were significantly upregulated to 4.99, 3.31, 2.75, 15.38, 11.48 in nonulcerative IC patients, but decreased to 0.04, 0. 29, 0. 09, 0.11, 0.34 in ulcerative IC patients （P^0.01）. The up-regulation of UP III-δ4 was more prominent than that of UP III： 26. 5 fold versus 5. 6-fold compared to the median values of normal subjects. Conclusion Although the clinical implications of the over-expression of UP III and III-δ4 in non ulcerative IC bladders remain to be clarified, from the diagnostic viewpoint, UP III-δ4 is a po tential marker for identifying nonulcerative IC.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2011年第6期399-402,共4页
Chinese Journal of Urology
