摘要
目的:研究尿激酶型纤溶酶原激活剂(uPA)及其抑制剂(PAI-1)在舌鳞癌(TSCC)中的表达及其与临床病理参数的关系。方法:应用链霉卵白素-生物素复合体方法检测uPA及PAI-1在74例TSCC组织和15例癌旁正常黏膜中的表达。以SPSS10.0软件包对数据进行统计学处理,Kappa一致性法分析uPA和PAI-1表达的相关性,χ2检验分析两者的表达水平与舌癌临床指标的关系,连续校正法检验两者联合表达与颈淋巴结转移间的关系。结果:TSCC组织中uPA和PAI-1阳性表达率分别为71.6%(53/74)和75.7%(56/74)。uPA的表达水平与临床分期、颈淋巴结转移相关,与肿瘤病理学分级无关;PAI-1与临床分期、颈淋巴结转移相关,与肿瘤病理学分级无关。uPA和PAI-1同时阳性表达组,颈淋巴结转移率显著高于同时阴性表达组(P<0.05)。结论:uPA和PAI-1表达在TSCC中明显升高,并与TSCC的颈淋巴结转移密切相关。
PURPOSE： To study the expressions and clinical significance of urokinase-type plasminogen activator（uPA） and its inhibitor（PAI-1） in tongue squamous cell carcinoma（TSCC）.METHODS： The expression of uPA and PAI-1 at protein level was examined in 74 TSCC and 15 normal peripheral tissues around cancer（as control） by strep avidin-biotin complex（SABC） immunohistochemical assay.SPSS10.0 software package was used to analyze the correlation of uPA and PAI-1 expression and related clinicopathological parameters.RESULTS： The rates of the positive expression in TSCC of uPA and PAI-1 were 71.6%（53/74） and 75.7%（56/74）,respectively.There was significant correlation between the level of uPA expression and TNM stage or cervical lymph node status,between the level of PAI-1 expression and T stage cervical lymph node status.Pathological grade was not significantly related to the level of uPA or PAI-1 expression.TSCC cases with positive expression of uPA and PAI-1 revealed significantly higher tendency to cervical lymph node metastasis than those with negative expression of uPA and PAI-1（P0.05）.CONCLUSIONS： The expression of uPA and PAI-1 are increased in TSCC.uPA and PAI-1 may contribute significantly to TSCC invasion and metastasis.
出处
《中国口腔颌面外科杂志》
CAS
2011年第3期223-226,共4页
China Journal of Oral and Maxillofacial Surgery
基金
辽宁省科技计划项目(2005225013-1)~~
关键词
尿激酶型纤溶酶原激活剂
舌肿瘤
鳞状细胞癌
免疫组织化学
Urokinase-type plasminogen activator
Tongue neoplasm
Squamous cell cancer
Immunohistochemistry
