摘要
目的:检测非小细胞肺癌(Non-small celllung cancer,NSCLC)组织中核酸内切酶Dicer、增殖细胞核抗原(Proliferating cell nuclear antigem,PCNA)蛋白的表达,探讨它们的相关性及临床意义。方法:应用免疫组织化学SP法,检测Dicer和PCNA在57例肺癌组织和16例癌旁正常肺组织表达情况,并结合肺癌的临床病理特征进行分析。结果:57例NSCLC Dicer蛋白失表达率50.9%,16例癌旁正常肺组织中为37.5%,二者差异无统计学意义(P>0.05);Dicer蛋白在肺腺癌失表达率为66.7%,鳞癌中为37.0%,二者差异有统计学意义(P<0.05);Dicer蛋白在高分化癌组(33.3%)失表达率低于低分化癌组(76.5%),二者差异有统计学意义(P<0.05);而与pTNM分期、淋巴结转移以及年龄、性别无关(P>0.05);NSCLC癌组织中Dicer高表达和低表达的PCNA平均指数分别为(37.61±20.31)%、(48.32±25.34)%(P>0.05)。结论:Dicer蛋白失表达可能参与肺腺癌的发生发展过程,影响肺肿瘤细胞的分化方向,没有直接影响PCNA蛋白表达。
Objective： To investigate the expression and significance of Dicer, PCNA protein in patients with non-small cell lung cancer. Methods ： The expressions of Dicer protein and PCNA protein and the clinico-pathologic features of lung cancer in 57 cases of lung cancer and 16 cases of normal lung tissue were analyzed statistically by immunohistochemical SP method. Resu/ts：The loss of expression of Dicer protein in 57 cases of lung carcinoma was 50.9% ,while in 16 cases of normal lung tissues the loss was 37.5%（P〉0.05 ）. The loss of expression of Dicer protein in lung adenocarcinoma was 66.7% ,while the loss was 37.0% in squamous carcinoma,which was statistically significant （ P〈0.05 ） between the two pathological types. The loss of expression of Dicer protein in high differentiation carcinoma （ 33.3% ） was lower than in low differentiation carcinoma （ 76.5% ）, which was statistically significant （ P〈0.05 ） between high and low differentiation cancers.However,no correlation was found between pTNM stages,lymph node metastasis,age and gender （ P〉0.05 ）. The mean index of proliferating cell nuclear antigen （ PCNA ） in low and high level expression of Dicer were（48.32 ± 25.34）% and （37.61 ± 20.31 ）% respectively （P〉0.05）. Conclusion：The loss of expression of Dicer protein may take part in the onset and process of lung adenocarcinoma;it influences the direction of differentiation of the lung carcinoma cell and is not directly linked to the expression of PCNA protein.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2011年第4期466-469,共4页
Journal of Chongqing Medical University
关键词
DICER
癌细胞增殖
非小细胞肺癌
免疫组化
dicer protein
cancer cell proliferation
non-small cell lung cancer
immunohistochemistry
