摘要
目的 观察不同浓度ｂｃｌ２ 癌基因反义硫代磷酸寡脱氧核苷酸（ＡＳＰＳＯＤＮ，ＡＳＰＯ） 对ＨＬ６０ 细胞凋亡的诱导作用。方法 ＡＳＰＯ与ＨＬ６０ 细胞共培养后，用吖啶橙（ＡＯ）染色法，流式细胞仪ＤＮＡ倍体分析，电镜观察、ＤＮＡ片段电泳等方法进行观察。结果 ＡＳＰＯ组与正义组比较能特异诱导ＨＬ６０ 细胞的凋亡，且于培养２４ 小时即可出现，此时ＡＳＰＯ５ 、１０、２０ μｍｏｌ／Ｌ浓度各组的凋亡率分别为：（９．７ ±２ ．２）％ 、（１８．３±３．０） ％ 和（２３．４ ±２ ．８）％ 。作用强度随ＡＳＰＯ浓度的增加而增大；ＡＳＰＯ５、１０ 、２０μｍｏｌ／Ｌ各组在作用７２ 小时的凋亡率分别为（９．６ ±３ ．０）％ 、（２９．６ ±４．０） ％ 、（３６ ．３ ±３．７）％ ，其中ＡＳＰＯ５ μｍｏｌ／Ｌ组以４８ 小时的凋亡率最高。结论 ＡＳＰＯ 通过封闭ｂｃｌ２ 的ｍＲＮＡ 能特异地诱导ＨＬ６０ 细胞的凋亡，且具有浓度依赖性。
Objective To observe the effects of different bcl 2 antisense phosphorothioate oligodeoxynucleotide (ASPO) concentrations in inducing apoptosis of HL60 cells. Methods Signs of apoptotic cells were detected by acridine orange staining, transmission electron microscope, flow cytometry and DNA fragment electrophoresis. Results In presence of ASPO, the apoptosis of HL60 cells could be induced after 24 hours of incubation. The apoptosis rate at ASPO 5, 10 and 20 μmol/L were (9.67±2.16)%, (18.25±3.01)% and (23.42±2.75)% respectively. There was a statistically significant increase in apoptosis as the dose of ASPO increased. After incubation for 72 hours, the apoptotic rates of the 3 different ASPO concentration groups were (9.58±2.96)%, (29.58±4.04)% and (36.33±3.66)% respectively, of which the apoptic rate of HL60 cells incubated with ASPO 5 μmol/L was highest at 48 hours. Conclusion Bcl 2 ASPO can specifically induce apoptosis of HL60 cells. Its effect depends upon the concentration of ASPO.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1999年第4期268-271,共4页
Chinese Journal of Pathology
基金
福建省科委科技项目基金
