摘要
目的:探讨HOXA10基因启动子区甲基化与卵巢上皮性癌(卵巢癌)的关系。方法:运用甲基化特异性聚合酶链反应(MSP)法检测29例卵巢癌标本及对照组16例正常卵巢组织中HOXA10基因启动子甲基化状态,并分析HOXA10基因甲基化状态与不同临床病理特征的联系。结果:检测到17例(58.62%)卵巢癌组织HOXA10基因启动子区发生低甲基化改变,另外4例甚至为完全去甲基化改变;而仅有4例(25.00%)正常卵巢组织HOXA10基因启动子区发生低甲基化(P<0.05)。HOXA10低甲基化阳性率随淋巴转移而增高(P<0.05)。不同年龄、瘤体大小、WHO病理分级、FIGO临床分期和HOXA10基因低甲基化未见明显相关性(P>0.05)。结论:HOXA10基因启动子区低甲基化为频发事件,提示与卵巢癌患者的不良预后相关,可作为卵巢癌判断预后的生物学标志物。
Objective： This study was aimed to investigate the promoter hypomethylation status of the HOXA10 genes in ovarian cancer and its correlation with the clinicopathologic characteristics.Methods： Promoter hypomethylation was evaluated by MSP（methylation-specific polymerase chain reaction） in 29 cases of ovarian cancer tissue specimens and 16 cases of normal control.The relationship between clinicopathologic parameters and the hypomethylation status was evaluated.Results： 17 cases（58.62%） of ovarian cancer showed promoter hypomethylation of HOXA10,even another 4 cases had complete demethylation;while only 4 cases of normal control had hypomethylation（25.00%）（P0.05）.The frequence of hypomethylation increased with positive lymph node metastasis（P0.05）.There were no significant associations bewteen the hypomethylation frequencies for HOXA10 gene and patient age,size of tumor,FIGO stages and histological grades（P0.05）.Conclusions： HOXA10 gene promoter hypomethylation is a frequent epigenetic event in ovarian carcinoma and has a significant correlation with the poor prognosis,so it could be a biological marker for ovarian cancer.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2011年第5期354-357,共4页
Journal of Practical Obstetrics and Gynecology
关键词
卵巢上皮性癌
同源盒基因
低甲基化
甲基化特异性聚合酶链反应
Epithelial ovarian cancer
Homeobox gene
Hypomethylation
Methylation specific polyerase chain reaction
