摘要
目的 研究全脑缺血再灌注损伤后脑线粒体膜流动性及Ｎａ＋ －Ｋ＋ ＡＴＰａｓｅ的变化及低温的影响。方法 健康家犬１６ 只随机分为三组：Ａ组（ｎ＝ ４），非缺血对照组；Ｂ组（ｎ＝ ６），心脏停跳复苏后常温常规治疗组；Ｃ组（ｎ＝ ６），心脏骤停复苏后，低温液灌注组。Ｂ、Ｃ两组动物心脏停跳１８ 分钟，复苏后治疗观察８ 小时，Ａ 组动物完成手术操作观察８小时，处死动物取脑组织测定线粒体膜流动性、Ｎａ＋ －Ｋ＋ ＡＴＰａｓｅ 活性和ＭＤＡ含量。结果 Ｂ组动物脑线粒体膜微粘度和ＭＤＡ明显高于正常对照组，浅低温治疗后，脑线粒体膜微粘度明显降低，Ｎａ＋ －Ｋ＋ ＡＴＰａｓｅ活性明显升高，ＭＤＡ 含量降低。结论 脑缺血再灌注可致脑线粒体膜功能损害，浅低温可促进脑线粒体膜功能的恢复。
Objective To study the effects of hypothermia on the mitochondral membrane fluidity,Na + K + ATPase in canine brain following cardiac arrest and resuscitation.Methods Sixteen dogs were divided randomly into three groups:group A (n=4),non ischemic control;group B (n=6),cardiac arrest and standard resuscitation;group C (n=6),cardiac arrest and hypothermia resuscitation.The duration of cardiac arrest was 18 min and the reperfusion time was 8h in group B and C.The fluidity and Na + K + ATPase activity of brain mitochondral membrane,content of MDA in brain tissue were measured.Results Compared with those of group A,the fluidity and Na + K + ATPase activity of brain mitochondral membrane decreased,and the content of MDA in brain tissue increased significantly in group B (P<0.01).The hypothermia inhibited all the above changes significantly(P<0.05).Conclusion The membrane activity is damaged during cardiac arrest and resuscicitation and mild hypothermia may improve its restoration.
出处
《中华麻醉学杂志》
CSCD
北大核心
1999年第8期468-470,共3页
Chinese Journal of Anesthesiology
