摘要
为了探讨植物提取物对香蕉采后病害的防治效果,以香蕉果实为材料,采用菌丝生长速率方法测定不同剂量(0～40μL)的茶树油(TTO)及其主要成分(4-松油烯醇、α-松油醇、γ-松油烯和1,8-桉叶油素)对香蕉炭疽病菌的离体抗菌活性,并用TTO及其主要成分熏蒸处理接种炭疽病菌的香蕉果实,测定果实常温贮藏(25°C,相对湿度75%)的发病情况和TTO处理对接种果实防御酶活性的影响。结果表明,TTO、4-松油烯醇和α-松油醇均能显著抑制离体炭疽病菌的菌丝生长,质量浓度越高,抑制效果越明显;1.25g/LTTO处理降低了接种炭疽病菌果实的发病程度,0.32g/L4-松油烯醇和α-松油醇处理对接种果实病斑直径的抑制效果显著好于TTO。另外,1.25g/LTTO处理提高了接种果实过氧化物酶(POD)和苯丙氨酸解氨酶(PAL)的酶活性,抑制了多酚氧化酶(PPO)酶活性。初步研究表明TTO熏蒸处理在控制香蕉果实采后腐烂和提高果实抗病性上具有应用可行性,4-松油烯醇和α-松油醇可能是其主要活性成分。
The effects of natural plants extracts against banana postharvest disease Colletotrichum musae was evaluate The antifungal activity of 0-40μL TTO（tea tree oil） and its main components （Terpinenol-4, α-Terpineol, γ-Terpine and 1,8-Cineole） against colony growth of C. musae in vitro and their effects on disease intensity and defense-relat（ enzymes of banana fruits inoculated with C. musae were determined at 25℃ and 75% relative humidity. Results show（ that fumigation of TTO, Terpinenol-4 and a-Terpineol could significantly inhibit C. musae growth in vitro. In additio the antifungal activity of TTO and its main components could be greatly enhanced with higher levels. Compared wi non-treated fruits, 1.25 g/L TTO fumigation reduced lesion diameter of inoculated banana fruits, but less than 0.32 Terpinenol-4 or α-Terpineol. Moreover. 1.25 g/L TTO fumigation induced significant increase in peroxidase（POD）, al phenylalanine ammonia-lyase（PAL）, but decreased polyphenoloxidase（PPO） activity. The preliminary study sugge, that TTO fumigation is feasible on fresh-keeping and increasing of disease resistance in postharvest banana frui＇ meanwhile, Terpinenol-4 and a-Terpineol are primary antifungal activities of TTO.
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2011年第5期378-384,共7页
Transactions of the Chinese Society of Agricultural Engineering
基金
中央级公益性科研院所基本科研业务费专项开放项目(SSCRI200906)
关键词
病害
农产品
熏蒸
香蕉
茶树油
活性成分
diseases, agricultural products, fumigation, banana, tea tree oil（TTO）, active component
