摘要
采用ELISA双抗体夹心法和3H－TdR释放法分别对176例各种肝病患者及36例正常人血清TNFα和sTNFα进行测定。结果发现各种肝病患者血清TNFα和sTNFαR均明显高于正常对照组（P＜0．05），TNFα和sTNFαR呈明显正相关。PHC和CAH患者血清sTNFαR1／sTNFαR2比值明显升高；而MHC和CPH患者血清sTNFαR1/sTNFαR2比值则明显减小（P＜0．05）。疗效较差、病情较重、炎症活动和肿瘤转移时血清TNFα和sTNFαR水平明显升高，提示TNFα参与了肝细胞损伤的病理过程。检测患者血清TNFα和sTNFαR不但能判断病情的严重程度，且能在一定程度上对疾病分期、疗效及预后等做出评估，并为临床诊断和治疗提供有价值的参考指标。
The levels of tumor necrosis factor-a(TNFa)and souble TNFa receptor(sTNFaR) were analysed by an enzyme-linked immunosorbent assay and radioimmunoassay in 176 cases of patients with liver diseases and 36 healthy adults served as control. The results showed that serum TNFa and sTNFaR levels in liver diseases group were significantly higher than those of healthy controls (P < 0.05 ~ 0.005).There was an obvious opitive correlation between TNFa and sTNFaR. The values of aTNFaR1/sTNFaR2in primary hepatic carcinoma and chronic active hepatitis B were obviously increased and in metastatic hepatic carcimoma and chronic persisting hepatitis B wer obviously decreased (P<0. 05). The levels of TNFa and sTNFaR correlated with clinical period, activity of hepatitis and metastasis of hepatoma. These findings show that TNFa participates in the activity process of liver diseases. Therefore, it is concluded that detection of serum TNFa and sTNFaR may reflect the severe of liver diseases and,to a certain degree, can be used to evaluate the therapeutic effect and be helpful to assess the prognosis of the diseases and provide reference valuable mark for clinical diagnosis and therapy as well.
出处
《临床肝胆病杂志》
CAS
北大核心
1999年第3期179-182,共4页
Journal of Clinical Hepatology
