摘要
用钙离子沉淀法制备猪肝细胞微粒体Ｐ４５０ 进行了体外代谢隐丹参酮的研究。用Ｌｏｗｒｙ 法测定肝微粒体蛋白含量平均为２ ．４６０ ±０ ．６９９ ｍｇ／ ｍ Ｌ；Ｏｍ ｕｒａ 法测定猪肝微粒体Ｐ４５０ 含量平均为０ ．１７６ ±０ ．０５３ ｎ ｍｏｌ／ ｍ ｇ 。从Ｐ４５０加还原型辅酶Ⅱ的体外代谢系统中分离纯化了２ 个主要代谢产物，经光谱分析鉴定为丹参酮ⅡＡ 与１羟基丹参酮ⅡＡ，并比较了隐丹参酮在猪体内外的代谢差异。
The pig liver microsomes P 450 were prepared by means of calcium ion precipitation in order to study in vitro metabolic transformation of Cryptotanshinone. The protein content of the liver microsomes was 2.460±0.66 mg/mL (n=5) by means of the Lowry's method; The average content of the liver microsomes P 450 was 0.176±0.053 nmol/mg ((n=5) by means of the Omura's method. The major metabolites were isolated by CC and preparative TLC, and identified as tanshinone Ⅱ A and 1 hydroxy tanshinone Ⅱ A by UV, EI MS and 1 HNMR spectral analysis. The difference of the metabolism profiles of Cryptotanshinone in vivo and in vitro was also compared.
出处
《中国兽医科技》
CSCD
1999年第11期10-13,共4页
Chinese Journal of Veterinary Science and Technology
基金
国家"九五"科技攻关子专题
中国农业科学院科技基金
