摘要
目的:通过EDU免疫荧光法观察整合素连接激酶(Integrin-Linked Kinase,ILK)在新生大鼠心肌细胞内高表达后对心肌细胞DNA合成的影响。方法:取新生(1-3天内)大鼠原代心肌细胞,培养72小时后随机分为正常对照组、ILK转染组。对照组转染重组腺病毒载体(adeno-GFP),ILK组转染重组腺病毒载体+ILK基因(adeno-ILK)。转然成功后48小时将两组心肌细胞分别通过5-乙基-2'-脱氧尿嘧啶核苷(EDU)免疫荧光法测定心肌细胞DNA合成。结果:ILK转染组心肌细胞内DNA合成较对照组明显增加(P<0.05)。结论:ILK高表达具有促进新生大鼠心肌细胞的DNA合成的能力。
To investigate the effects of over-expressed integrin-linked kinase on rat neonatal DNA synthesis of cardiomyoeytes by EDU immunofluorescence assay. Methods： Primary neonatal rat （1-3 days old） cardiomyocytes were cultured in vitro and randomly divided into two groups 72 h after isolation： infected by either adeno-GFP （control group） or adeno-GFP containing adeno-lLK （ILK group）. 48h after infection, DNA synthesis of cardiomyocytes in two groups were determined by 5-ethynyl-2-deoxyuridine（EdU） immunofluorescence assay. Results： Compared with the control groups, DNA synthesis of cardiomyocytes increased dramatically in ILK groups （P 〈0.05）. Conclusion： The over-expression of ILK could promote Rat Neonatal DNA synthesis of cardiomyocytes.
出处
《现代生物医学进展》
CAS
2011年第10期1858-1860,共3页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81070195)
