摘要
目的:观察消痰散结方对人胃癌MNK-45荷瘤鼠糖酵解相关蛋白己糖激酶-Ⅱ(HK-Ⅱ)表达的影响,探讨该方抗肿瘤机理。方法:30只小鼠随机分为生理盐水组、消痰散结方组和希罗达组,每组10只。建立人胃癌MNK-45皮下移植瘤模型。灌胃治疗六周后,称取各组瘤质量,计算抑瘤率和瘤体积。酶联免疫吸附测定法检测荷瘤鼠血清中HK-Ⅱ的水平;免疫组化方法检测瘤组织中HK-Ⅱ蛋白的表达;结果:消痰散结方可明显抑制裸鼠人胃癌MNK-45皮下移植瘤生长,下调荷瘤鼠血清及瘤组织中HK-Ⅱ表达水平。结论:降低荷瘤鼠血清和组织中HK-Ⅱ的表达,抑制荷瘤鼠机体糖代谢过程可能是该方抗肿瘤作用的机制之一。
To explore the antitumor effect of eliminating phlegm recipe on MNK-45 human gastric tumor-bearing mice. Methods： Thirty Kunming mice were randomly divided into normal saline （NS） group, Xiaotan Sanjie Recipe group and Xeloda group, ten for ench.Tumor-bearing models were established by injecting human gastric cancer MKN-45 injected into the subcutaneous site of BALB/c/-nu/nu mice. The mice were administered with medicine for six weeks. Weight of tumor xenografts were measured. tumor inhibiton rate and tumor volume were calculated. The expression of HK- Ⅱ was tested by enzyme-linked immunosorbent assay and immunohistochemistry. Results： Xiaotan sanjie Recipe group significantly inhibits the growth of the transplanted tumor and decrease The expression of ilK- Ⅱ in blood and tumor tissue. Conclusion： Xiaotan sanjie Recipe Could decrease the expression of ilK-Ⅱ in blood and tumor tissue,It may be one of the mechanisms of its in inhibiting the tumor growth.
出处
《现代生物医学进展》
CAS
2011年第10期1841-1843,1837,共4页
Progress in Modern Biomedicine
关键词
消痰散结方
肿瘤
糖酵解
小鼠
Xiaotan Sanjie Recipe
Tumor
Glycometabolism
Mice
