摘要
通过土壤单因子Pb胁迫盆栽试验和铅锌尾矿污染土壤掺比试验研究了不同土培条件下Pb对马蔺(Iris lacteavarchinensis)生长、体内重金属元素积累以及叶绿体超微结构的影响。结果表明:2种不同土壤Pb胁迫下马蔺株高、根长、叶数以及地上、地下部生物量均随Pb浓度的增加呈先增后降的趋势,其中,土壤单Pb胁迫下,低于500 mg/kg Pb胁迫(T1—T3)均增加了马蔺的株高和根长,仅800mg/kg高Pb浓度处理时马蔺生物量才出现轻微下降(P>0.05);与单一土壤Pb胁迫相比,铅锌尾矿污染土壤掺比试验中铅锌尾矿含量低于一定浓度下对马蔺株高、根长生长同样也具有促进效应,只有100%尾矿污染土壤处理下马蔺地上部和根系生物量下降显著(P<0.05)。马蔺地上部和根系Pb含量均随土壤中Pb浓度和尾矿含量的增加而增加,800mg/kg土壤单Pb胁迫下为340.6、1700.02 mg/kg,100%尾矿污染土壤处理下为126.9、1725 mg/kg。Pb单独胁迫下马蔺地上部Zn含量随Pb含量的增加逐渐下降,Zn和Pb的吸收表现出一定的拮抗效应;而不同掺比污染土壤胁迫下马蔺地上部和根系Zn积累同Pb一样,均随土壤中Pb、Zn浓度的增加而增加,Zn和Pb的吸收表现为一种协同效应。而2种不同土壤Pb胁迫下马蔺对Cu、Cd的吸收均相对较少。研究中,800mg/kg Pb胁迫处理和50%尾砂土壤胁迫处理下马蔺叶片叶绿体双层被膜及内部基粒和类囊体结构与对照相比变化不大。综上表明,马蔺对重金属Pb有较强的耐受性,具备修复Pb污染土壤的潜在能力。
Effects of Pb on the growth, the uptake and accumulation of lead （Pb）, zinc （Zn）, copper（Cu）, cadmium （Cd） and chloroplast ultrastructure of Iris lactea var. chinensis were studied by pot-culture experiments of the soil treated with single Pb and that treated with mixed Pb and Zn railings. The results showed that the seedling height, root length, leaf number, shoot and root biomass of I. lactea vat chinensis were all increased first and then dropped in two different soil treatments. The seedling height and root length of the plant were increased under lower single Pb treatment（ ≤500 mg/kg ） and slightly＇deereased（P〉O. 05 ） under 800 mg/kg Pb in single Pb experiment. Compared with soil of single Pb stress, the soil mixed Pb and Zn tailings also displayed a promoting effect on the height and root length of Iris lactea var. chinensis when Pb and Zn tailings in soil below a certain concentration. Only under the treatment of 100% tailing the shoot and root bioinass of L lactea vat chinensis were decreased significantly compared with that of control（P〈O. 05）. The Pb contents both in shoots and roots were enhanced with increasing Pb and tailing concentrations in the soil. The leves of Pb in shoots and roots reached 340.6 mg/kg and 1700.02 mg/kg, respectively, in 800 mg/kg Pb treatment, and which reached 126.9mg/kg and 1725 mg/kg, respectively, in 100% railings treatment. The contents of Zn accumulated in shoots of the plant were decreased with an increase of Pb under single Pb stress, expressing as an antagonistic effect. However, the absorption of Zn was increased as the increasing of Pb accumulation in mixed contaminated railings stress, expressing as a synergistic effect. In contrast, the uptake of Cu and Cd are relatively small. In this study, the chloroplast double membrane and its internal grana and thylakoid structure were almost without change compared with the control under 800 mg/kg Pb and 50% tailings stress. These results indicated that I. lactea vat chinensis was a stron
出处
《生态学报》
CAS
CSCD
北大核心
2011年第12期3350-3357,共8页
Acta Ecologica Sinica
基金
国家自然科学基金项目(30771520)
江苏省科技厅社会发展项目(BE2008670)
江苏省自然基金资助项目(BK2010477)
关键词
PB污染
马蔺
积累
叶绿体超微结构
lead （Pb） pollution
Iris lactea var chinensis
accumulate
ultrastructure of chloroplast
