摘要
目的 观察颌面枪弹伤局部软组织中白细胞介素 (interleukin ,IL) 6、8(IL 6、IL 8)、肿瘤坏死因子 (tumornecrosisfactor alpha,TNF α)的表达情况 ,分析前炎症细胞因子对伤口愈合的影响。方法应用免疫组织化学方法检测 3种前炎症细胞因子在狗下颌枪弹伤模型伤口局部软组织中的表达 ,结合伤口组织病理学变化分析创伤愈合过程中前炎症细胞因子的作用机理。结果 枪弹伤后IL 6、IL 8、TNF α在伤口局部开始高表达 ,近、远伤区有不同 ,远伤区呈单峰 ,近伤区呈双峰 ,IL 6、IL 8特别明显 ;不同的细胞因子表达高峰时间点有所不同 ;3种因子表达的组织细胞定位基本相同 ,主要为成纤维细胞、内皮细胞、单核细胞等。结论 前炎症细胞因子在伤后软组织中的高表达对创伤炎症反应影响较大 ,是调控伤后早期炎症水平的主要因素 ;它们促进成纤维细胞、内皮细胞及炎细胞功能 ,刺激与调控这些创伤细胞分泌其他细胞因子 ,从而对创伤组织愈合过程产生重要的影响。
Objective To study the relationship between proinflammentory cytokines and wound healing in local area Methods Interleukin(IL) 6, IL 8 and tumor necrosis factor alpha (TNF α) expression in local soft tissues after lower jaw gunshot wound were assessed by using immunohisto chemistry, and pathological methods Results High expressions of these cytokines were observed in local soft tissues of wounds after the gunshot The cytokines expressions were different between the area near the bullet path and that far from the path There was only one peak of the cytokines concentrations in the area far from the bullet path, while there were two peaks of the cytokines concentrations in the area near the bullet path, specially IL 6, IL 8 There were time differences for some different cytokines to reach the peaks of the their concentrations In wound situ, the fibroblasts, mononuclear phagocytes and endothelial cells expressed IL 6, IL 8 and TNF α after the firearm Conclusion These results suggest that proinflammatory cytokines play an important role in regulating inflammatory cytokine reactions of local injury tissues They might improve the functions of fibroblasts, mononuclear phagocytes and endothelial cells and stimulate wound cells to produce and release other cytokines
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
1999年第5期278-280,共3页
Chinese Journal of Stomatology
