摘要
利用来自Bacillus subtilis细菌的CspB基因(Gene ID:936224)构建了Ubiquitin启动子驱动的CspB基因植物表达载体PBPC-CspB-bar,以bar基因为抗性筛选标记,通过花粉管通道法将构建的表达载体转化到玉米自交系京501、京517和吉444,通过喷洒除草剂筛选得到60株草丁膦抗性植株,用PCR检测得到48株bar基因阳性植株,将获得的转基因植株进行CspB基因PCR鉴定,获得13株同时整合CspB和bar的转基因株系。
A plant expression vector PBPC-CspB-bar harboring CspB gene driven by corn ubiquitin promoter from the Bacillus subtilis bacteria（Gene ID：936224） was constructed.The bar gene as resistance selection marker,the recombinant plasmid was introduced into maize inbred lines Jing501,Jing517,and Ji444,using pollen-tube-pathway method,and 60 glufosinate-tolerant plants were obtained through herbicide screening.Then 48 transgenic plants were proved by PCR assays for bar gene.To further identify the transgenic plants,PCR analysis for CspB was conducted in the 48 bar gene positive plants.It was showed that both CspB and bar had been integrated into the maize genome in 13 transgenic lines.
出处
《作物杂志》
CAS
CSCD
北大核心
2011年第3期70-74,共5页
Crops
基金
国家转基因重大专项重点课题(2009ZX08003-009B)
北京市科委项目(Z090605006009014和Z09090501040902)
北京市农林科学院科研能力创新项目
