摘要
在对基因工程链霉菌Ｋ３的研究中，我们从其发酵液中分离到Ｋ３－Ｄ４、Ｋ３－Ｄ５、Ｋ３－Ｄ６３个化合物。通过ＵＶ、ＩＲ、ＭＳ、１Ｈ－ＮＭＲ、１３Ｃ－ＮＭＲ及ＨＭＢＣ等光谱分析确定Ｋ３－Ｄ４、Ｋ３－Ｄ５、Ｋ３－Ｄ６为３个新的异黄酮类化合物，分别为４′，５，７－三羟基－３′－硝基异黄酮、４′，５，７－三羟基－３′，５′－二硝基异黄酮、４′，７－二羟基－３′。
This thesis is on the study of lipophilic components of the genetic engeering streptomyces K3. The fermentation broth was adjusted to pH 3 and extracted with ethyl acetate. By means of Sephadex LH 20 chromatography, preparative TLC and RP MPLC, three compounds, K3 D4, K3 D5, K3 D6 respectively were isolated from the mixture of the extract. By extensive study of their UV, IR, MS, 1H NMR, 13 C NMR and HMBC spectra, the structure of K3 D4, K3 D5 and K3 D6 were elucidated. They are three novel isoflavonoids. K3 D4 was assigned to be 4H 1 Benzopyran 4 one, 5,7 dihydroxy 3 (3 nitro 4 hydroxy phenyl) ; K3 D5 was assigned to be 4H 1 Benzopyran 4 one, 5,7 dihydroxy 3 (3,5 dinitro 4 hydroxy phenyl) ; K3 D6 was assigned to be 4H 1 Benzopyran 4 one, 7 hydroxy 3 (3,5 dinitro 4 hydroxyphenyl).
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
1999年第4期254-257,299,共5页
Chinese Journal of Antibiotics
关键词
链霉菌K3
异黄酮
分离
结构鉴定
阿米卡星
Isoflavonoid
4H 1 Benzopyran 4 one, 5,7 dihydroxy 3 (3 nitro 4 hydroxyphenyl)
4H 1 Benzopyran 4 one, 5,7 dihydroxy 3 (3,5 dinitro 4 hydroxyphenyl)
4H 1 Benzopyran 4 one, 7 hydroxy 3 (3,5 dinitro 4 hydroxyphenyl)
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